Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes
Joint Authors
Goldberg, Anna Carla
Piccinato, Carla A.
Antonioli, Eliane
Cohen, Moisés
Ferretti, Mario
Nader, Helena Bonciani
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-26
Country of Publication
Egypt
No. of Pages
11
Abstract EN
Bone marrow mesenchymal stem cells (BM-MSCs) are considered a good source for cellular therapy in cartilage repair.
But, their potential to repair the extracellular matrix, in an osteoarthritic environment, is still controversial.
In osteoarthritis (OA), anti-inflammatory action and extracellular matrix production are important steps for cartilage healing.
This study examined the interaction of BM-MSC and OA-chondrocyte on the production of hyaluronan and inflammatory cytokines in a Transwell system.
We compared cocultured BM-MSCs and OA-chondrocytes with the individually cultured controls (monocultures).
There was a decrease in BM-MSCs cell count in coculture with OA-chondrocytes when compared to BM-MSCs alone.
In monoculture, BM-MSCs produced higher amounts of hyaluronan than OA-chondrocytes and coculture of BM-MSCs with OA-chondrocytes increased hyaluronan production per cell.
Hyaluronan synthase-1 mRNA expression was upregulated in BM-MSCs after coculture with OA-chondrocytes, whereas hyaluronidase-1 was downregulated.
After coculture, lower IL-6 levels were detected in BM-MSCs compared with OA-chondrocytes.
These results indicate that, in response to coculture with OA-chondrocytes, BM-MSCs change their behavior by increasing production of hyaluronan and decreasing inflammatory cytokines.
Our results indicate that BM-MSCs per se could be a potential tool for OA regenerative therapy, exerting short-term effects on the local microenvironment even when cell:cell contact is not occurring.
American Psychological Association (APA)
Antonioli, Eliane& Piccinato, Carla A.& Nader, Helena Bonciani& Cohen, Moisés& Goldberg, Anna Carla& Ferretti, Mario. 2015. Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes. Stem Cells International،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076312
Modern Language Association (MLA)
Antonioli, Eliane…[et al.]. Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes. Stem Cells International No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1076312
American Medical Association (AMA)
Antonioli, Eliane& Piccinato, Carla A.& Nader, Helena Bonciani& Cohen, Moisés& Goldberg, Anna Carla& Ferretti, Mario. Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1076312
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076312