Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes

Abstract EN

Bone marrow mesenchymal stem cells (BM-MSCs) are considered a good source for cellular therapy in cartilage repair.

But, their potential to repair the extracellular matrix, in an osteoarthritic environment, is still controversial.

In osteoarthritis (OA), anti-inflammatory action and extracellular matrix production are important steps for cartilage healing.

This study examined the interaction of BM-MSC and OA-chondrocyte on the production of hyaluronan and inflammatory cytokines in a Transwell system.

We compared cocultured BM-MSCs and OA-chondrocytes with the individually cultured controls (monocultures).

There was a decrease in BM-MSCs cell count in coculture with OA-chondrocytes when compared to BM-MSCs alone.

In monoculture, BM-MSCs produced higher amounts of hyaluronan than OA-chondrocytes and coculture of BM-MSCs with OA-chondrocytes increased hyaluronan production per cell.

Hyaluronan synthase-1 mRNA expression was upregulated in BM-MSCs after coculture with OA-chondrocytes, whereas hyaluronidase-1 was downregulated.

After coculture, lower IL-6 levels were detected in BM-MSCs compared with OA-chondrocytes.

These results indicate that, in response to coculture with OA-chondrocytes, BM-MSCs change their behavior by increasing production of hyaluronan and decreasing inflammatory cytokines.

Our results indicate that BM-MSCs per se could be a potential tool for OA regenerative therapy, exerting short-term effects on the local microenvironment even when cell:cell contact is not occurring.