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Performance of LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis in HIV Positive Individuals in Addis Ababa, Ethiopia
Joint Authors
Getachew, Konjit
Abebe, Tamrat
Kebede, Abebaw
Melkamu, Getachew
Mihret, Adane
Source
Tuberculosis Research and Treatment
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Despite its lower sensitivity, smear microscopy remains the main diagnostic method for pulmonary tuberculosis (PTB) in resource-limited countries as TB culturing methods like LJ (Lowenstein-Jensen) are expensive to use as a routine base.
This study aimed to evaluate the performance of LED-FM for the diagnosis of PTB in HIV positive individuals.
Methods.
Cross-sectional study was conducted in Zewditu Memorial Hospital and Teklehaimanot Health Center HIV/ART clinics in Addis Ababa, Ethiopia.
Each sample was stained with ZN and Auramine O staining and examined with bright-field microscope and LED-FM microscope, respectively.
LJ culture was used as a reference.
Results.
Out of 178 study participants, twenty-four (13.5%) patients were confirmed as positive for MTB with LJ culture.
The yield of ZN microscopy and LED-FM in direct and concentrated sample was 3.9%, 8.4%, 6.2%, and 8.4%, respectively.
Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of direct ZN microscopy were 29.2%, 100%, 100%, and 90.1%, respectively, and of LED-FM microscopy in direct sputum sample were 62.5%, 100%, 100%, and 94.5%, respectively.
Conclusion.
LED-FM has better sensitivity for the diagnosis of PTB in HIV positive individuals as compared to conventional ZN microscopy.
LED-FM can be used as an alternative to conventional ZN microscopy.
American Psychological Association (APA)
Getachew, Konjit & Abebe, Tamrat& Kebede, Abebaw& Mihret, Adane& Melkamu, Getachew. 2015. Performance of LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis in HIV Positive Individuals in Addis Ababa, Ethiopia. Tuberculosis Research and Treatment،Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1078441
Modern Language Association (MLA)
Getachew, Konjit…[et al.]. Performance of LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis in HIV Positive Individuals in Addis Ababa, Ethiopia. Tuberculosis Research and Treatment No. 2015 (2015), pp.1-6.
https://search.emarefa.net/detail/BIM-1078441
American Medical Association (AMA)
Getachew, Konjit & Abebe, Tamrat& Kebede, Abebaw& Mihret, Adane& Melkamu, Getachew. Performance of LED Fluorescence Microscopy for the Diagnosis of Pulmonary Tuberculosis in HIV Positive Individuals in Addis Ababa, Ethiopia. Tuberculosis Research and Treatment. 2015. Vol. 2015, no. 2015, pp.1-6.
https://search.emarefa.net/detail/BIM-1078441
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078441