Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability
Joint Authors
Lin, Che-Tong
Huang, Yung-Kai
Lee, Lin-Wen
Hsieh, Sung-Chih
Tsao, Jeng-Ting
Lew, Wei-Zhen
Chan, Ya-Hui
Huang, Haw-Ming
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Medicine
Information Technology and Computer Science
Abstract EN
One of the causes of dental pulpitis is lipopolysaccharide- (LPS-) induced inflammatory response.
Following pulp tissue inflammation, odontoblasts, dental pulp cells (DPCs), and dental pulp stem cells (DPSCs) will activate and repair damaged tissue to maintain homeostasis.
However, when LPS infection is too serious, dental repair is impossible and disease may progress to irreversible pulpitis.
Therefore, the aim of this study was to examine whether static magnetic field (SMF) can attenuate inflammatory response of dental pulp cells challenged with LPS.
In methodology, dental pulp cells were isolated from extracted teeth.
The population of DPSCs in the cultured DPCs was identified by phenotypes and multilineage differentiation.
The effects of 0.4 T SMF on DPCs were observed through MTT assay and fluorescent anisotropy assay.
Our results showed that the SMF exposure had no effect on surface markers or multilineage differentiation capability.
However, SMF exposure increases cell viability by 15%.
In addition, SMF increased cell membrane rigidity which is directly related to higher fluorescent anisotropy.
In the LPS-challenged condition, DPCs treated with SMF demonstrated a higher tolerance to LPS-induced inflammatory response when compared to untreated controls.
According to these results, we suggest that 0.4 T SMF attenuates LPS-induced inflammatory response to DPCs by changing cell membrane stability.
American Psychological Association (APA)
Hsieh, Sung-Chih& Tsao, Jeng-Ting& Lew, Wei-Zhen& Chan, Ya-Hui& Lee, Lin-Wen& Lin, Che-Tong…[et al.]. 2015. Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability. The Scientific World Journal،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1078835
Modern Language Association (MLA)
Hsieh, Sung-Chih…[et al.]. Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability. The Scientific World Journal No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1078835
American Medical Association (AMA)
Hsieh, Sung-Chih& Tsao, Jeng-Ting& Lew, Wei-Zhen& Chan, Ya-Hui& Lee, Lin-Wen& Lin, Che-Tong…[et al.]. Static Magnetic Field Attenuates Lipopolysaccharide-Induced Inflammation in Pulp Cells by Affecting Cell Membrane Stability. The Scientific World Journal. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1078835
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1078835