High performance liquid chromatographic method for the determination of piroxicam, naproxen, diclofenac sodium, and mefenamic acid in bulk drug and pharmaceutical preparations
Joint Authors
Mahmud, Ruaa Muayyad
Dikran, Sarmad Bahjat
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 26, Issue 5 (31 May. 2018), pp.387-399, 13 p.
Publisher
Publication Date
2018-05-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Abstract EN
A simple accurate and rapid reversed phase high performance liquid chromatographic method has been developed and validated for the determination of four nonsteroidal anti-inflammatory drugs; Piroxicam(PX), Naproxen(NAP), Diclofenac sodium(DCL) and Mefenamic acid(MFNC) in their pure form and different commercial pharmaceutical formulation.
The separation was performed on a NUCLEODUR® 100-5 C18ec (250 × 4.6 mm i.d.; particle size 5 μm) column as stationary phase with a mobile phase comprising of acetonitrile: deionized water acidified with 1% acetic acid in gradient mode.
The flow rate was 1.5 mL.min-1 at the temperature 35 oC and detection was carried out at 264 nm.
Separation has been completed within 8 min.
The retention times of Piroxicam, Naproxen, Diclofenac sodium, and Mefenamic acid were 4.267 min, 4.785 min, 6.555min, and 7.683min respectively.
The linearity for Piroxicam was in the range of 3–200 μg.mL-1 and for Naproxen and Mefenamic acid was in the range 1-200 μg.mL-1 , while for Diclofenac sodium was in the range 1.5–200 μg.mL-1 .
The proposed method was successfully applied for the determination of these drugs in their pure form and different pharmaceutical preparations (tablets, capsules, and ampoule) and there is no interference with additives.
American Psychological Association (APA)
Dikran, Sarmad Bahjat& Mahmud, Ruaa Muayyad. 2018. High performance liquid chromatographic method for the determination of piroxicam, naproxen, diclofenac sodium, and mefenamic acid in bulk drug and pharmaceutical preparations. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 5, pp.387-399.
https://search.emarefa.net/detail/BIM-1093467
Modern Language Association (MLA)
Dikran, Sarmad Bahjat& Mahmud, Ruaa Muayyad. High performance liquid chromatographic method for the determination of piroxicam, naproxen, diclofenac sodium, and mefenamic acid in bulk drug and pharmaceutical preparations. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 5 (2018), pp.387-399.
https://search.emarefa.net/detail/BIM-1093467
American Medical Association (AMA)
Dikran, Sarmad Bahjat& Mahmud, Ruaa Muayyad. High performance liquid chromatographic method for the determination of piroxicam, naproxen, diclofenac sodium, and mefenamic acid in bulk drug and pharmaceutical preparations. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 5, pp.387-399.
https://search.emarefa.net/detail/BIM-1093467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 398-399
Record ID
BIM-1093467