Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study
Joint Authors
Henrich, Birgit
Hermann, Ilka
Di Giulio, Mara
Sivalingam, Sugi
Peters, Ulrike
Beikler, Thomas
Janda, Ralf
Rüttermann, Stefan
Köhrer, Karl
Deenen, René
Source
Advances in Materials Science and Engineering
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-13
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Purpose.
To introduce additional methods to detect and to quantify single pathogens in the complex biofilm formation on an antibacterial dental material.
Materials and Methods.
A conventional (ST) and an antibacterial dental composite (B) were manufactured.
In vitro: specimens were incubated with a mixture of early colonizers.
Bacterial adhesion was analyzed by TaqMan PCR after 8/24 h.
In situ: TaqMan PCR and 16S rRNA Next Generation Sequencing (NGS) were performed.
Results.
In vitro: after 8 h incubation, B was covered by 58.6% of the bacterial amount that was attached to ST.
After 24 h, the amount of attached bacteria to ST remained constant on ST only slightly lower on B.
In situ: after 8 h the amount of adhering A.
viscosus and S.
mitis was prominent on ST and reduced on B.
NGS revealed that S.
sanguinis, S.
parasanguinis, and Gemella sanguinis were the mainly attached species with S.
sanguinis dominant on ST and S.
parasanguinis and G.
sanguinis dominant on B.
Conclusions.
Initial biofilm formation was altered by B.
A shift between actinomycetes and streptococci was observed in situ.
TaqMan PCR and 16S rRNA NGS revealed comparable results in situ and demonstrated the usefulness of NGS to characterize complex bacterial communities.
American Psychological Association (APA)
Henrich, Birgit& Hermann, Ilka& Di Giulio, Mara& Köhrer, Karl& Deenen, René& Sivalingam, Sugi…[et al.]. 2016. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
Modern Language Association (MLA)
Henrich, Birgit…[et al.]. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
American Medical Association (AMA)
Henrich, Birgit& Hermann, Ilka& Di Giulio, Mara& Köhrer, Karl& Deenen, René& Sivalingam, Sugi…[et al.]. Reexamination In Vitro and In Situ of an Antibacterially Modified Experimental Dental Resin Composite with Molecular Methods: A Pilot Study. Advances in Materials Science and Engineering. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096277
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096277