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Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay
Joint Authors
Adewumi, Moses Olubusuyi
Faleye, Temitope Oluwasegun Cephas
Coker, Bamidele Atinuke
Nudamajo, Felix Yasha
Adeniji, Johnson Adekunle
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-20
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Recently, a cell-culture independent protocol for detection of enteroviruses from clinical specimen was recommended by the WHO for surveillance alongside the previously established protocols.
Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols.
Faecal samples were collected from sixty apparently healthy children in Ibadan, Nigeria.
Samples were resuspended in phosphate buffered saline, RNA was extracted, and the VP1 gene was amplified using WHO recommended RT-snPCR protocol.
Amplicons were sequenced and sequences subjected to phylogenetic analysis.
Fifteen (25%) of the 60 samples yielded the expected band size.
Of the 15 amplicons sequenced, 12 were exploitable.
The remaining 3 had electropherograms with multiple peaks and were unexploitable.
Eleven of the 12 exploitable sequences were identified as Coxsackievirus A1 (CVA1), CVA3, CVA4, CVA8, CVA20, echovirus 32 (E32), enterovirus 71 (EV71), EVB80, and EVC99.
Subsequently, the last exploitable sequence was identified as enterobacteriophage baseplate gene by nucleotide BLAST.
The results of this study document the first description of molecular sequence data on CVA1, CVA8, and E32 strains present in Nigeria.
The result further showed that species A enteroviruses were more commonly detected in the region when cell-culture bias is bypassed.
American Psychological Association (APA)
Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi& Coker, Bamidele Atinuke& Nudamajo, Felix Yasha& Adeniji, Johnson Adekunle. 2016. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
Modern Language Association (MLA)
Faleye, Temitope Oluwasegun Cephas…[et al.]. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
American Medical Association (AMA)
Faleye, Temitope Oluwasegun Cephas& Adewumi, Moses Olubusuyi& Coker, Bamidele Atinuke& Nudamajo, Felix Yasha& Adeniji, Johnson Adekunle. Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay. Advances in Virology. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1096770
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096770