Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma
Joint Authors
Tian, Fei
Li, Rui
Chen, Zhenzhu
Shen, Yanting
Lu, Jiafeng
Xie, Xueying
Ge, Qinyu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Lung cancer is the leading cause of cancer deaths.
Non-small-cell lung cancer (NSCLC) is the major type of lung cancer.
The aim of this study was to characterize the expression profiles of miRNAs in adenocarcinoma (AC), one major subtype of NSCLC.
In this study, the miRNAs were detected in normal, adjacent, and tumor tissues by next-generation sequencing.
Then the expression levels of differential miRNAs were quantified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
In the results, 259, 401, and 389 miRNAs were detected in tumor, adjacent, and normal tissues of pooled AC samples, respectively.
In addition, for the first time we have found that miR-21-5p and miR-196a-5p were gradually upregulated from normal to adjacent to tumor tissues; miR-218-5p was gradually downregulated with 2-fold or greater change in AC tissues.
These 3 miRNAs were validated by qRT-PCR.
Lastly, we predicted target genes of these 3 miRNAs and enriched the potential functions and regulatory pathways.
The aberrant miR-21-5p, miR-196a-5p, and miR-218-5p may become biomarkers for diagnosis and prognosis of lung adenocarcinoma.
This research may be useful for lung adenocarcinoma diagnosis and the study of pathology in lung cancer.
American Psychological Association (APA)
Tian, Fei& Li, Rui& Chen, Zhenzhu& Shen, Yanting& Lu, Jiafeng& Xie, Xueying…[et al.]. 2016. Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096878
Modern Language Association (MLA)
Tian, Fei…[et al.]. Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096878
American Medical Association (AMA)
Tian, Fei& Li, Rui& Chen, Zhenzhu& Shen, Yanting& Lu, Jiafeng& Xie, Xueying…[et al.]. Differentially Expressed miRNAs in Tumor, Adjacent, and Normal Tissues of Lung Adenocarcinoma. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096878
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096878