Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible Fungus Pleurotus ostreatus
Joint Authors
Qu, Jibin
Zhao, Mengran
Hsiang, Tom
Feng, Xiaoxing
Zhang, Jinxia
Huang, Chenyang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Noncoding RNAs (ncRNAs) have been identified in many fungi.
However, no genome-scale identification of ncRNAs has been inventoried for basidiomycetes.
In this research, we detected 254 small noncoding RNAs (sncRNAs) in a genome assembly of an isolate (CCEF00389) of Pleurotus ostreatus, which is a widely cultivated edible basidiomycetous fungus worldwide.
The identified sncRNAs include snRNAs, snoRNAs, tRNAs, and miRNAs.
SnRNA U1 was not found in CCEF00389 genome assembly and some other basidiomycetous genomes by BLASTn.
This implies that if snRNA U1 of basidiomycetes exists, it has a sequence that varies significantly from other organisms.
By analyzing the distribution of sncRNA loci, we found that snRNAs and most tRNAs (88.6%) were located in pseudo-UTR regions, while miRNAs are commonly found in introns.
To analyze the evolutionary conservation of the sncRNAs in P.
ostreatus, we aligned all 254 sncRNAs to the genome assemblies of some other Agaricomycotina fungi.
The results suggest that most sncRNAs (77.56%) were highly conserved in P.
ostreatus, and 20% were conserved in Agaricomycotina fungi.
These findings indicate that most sncRNAs of P.
ostreatus were not conserved across Agaricomycotina fungi.
American Psychological Association (APA)
Qu, Jibin& Zhao, Mengran& Hsiang, Tom& Feng, Xiaoxing& Zhang, Jinxia& Huang, Chenyang. 2016. Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible Fungus Pleurotus ostreatus. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097087
Modern Language Association (MLA)
Qu, Jibin…[et al.]. Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible Fungus Pleurotus ostreatus. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097087
American Medical Association (AMA)
Qu, Jibin& Zhao, Mengran& Hsiang, Tom& Feng, Xiaoxing& Zhang, Jinxia& Huang, Chenyang. Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible Fungus Pleurotus ostreatus. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097087
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097087