High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism
Joint Authors
Chen, Minjiang
Wei, Tingting
Wang, Dan
Xia, Huanhuan
Zhao, Liangcai
Ji, Jiansong
Zheng, Hong
Gao, Hong-Chang
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objective.
High glucose- (HG-) induced neuronal cell death is responsible for the development of diabetic neuropathy.
However, the effect of HG on metabolism in neuronal cells is still unclear.
Materials and Methods.
The neural-crest derived PC12 cells were cultured for 72 h in the HG (75 mM) or control (25 mM) groups.
We used NMR-based metabolomics to examine both intracellular and extracellular metabolic changes in HG-treated PC12 cells.
Results.
We found that the reduction in intracellular lactate may be due to excreting more lactate into the extracellular medium under HG condition.
HG also induced the changes of other energy-related metabolites, such as an increased succinate and creatine phosphate.
Our results also reveal that the synthesis of glutamate from the branched-chain amino acids (isoleucine and valine) may be enhanced under HG.
Increased levels of intracellular alanine, phenylalanine, myoinositol, and choline were observed in HG-treated PC12 cells.
In addition, HG-induced decreases in intracellular dimethylamine, dimethylglycine, and 3-methylhistidine may indicate a downregulation of methyl group metabolism.
Conclusions.
Our metabolomic results suggest that HG-induced neuronal cell death may be attributed to a series of metabolic changes, involving energy metabolism, amino acids metabolism, osmoregulation and membrane metabolism, and methyl group metabolism.
American Psychological Association (APA)
Chen, Minjiang& Zheng, Hong& Wei, Tingting& Wang, Dan& Xia, Huanhuan& Zhao, Liangcai…[et al.]. 2016. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
Modern Language Association (MLA)
Chen, Minjiang…[et al.]. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
American Medical Association (AMA)
Chen, Minjiang& Zheng, Hong& Wei, Tingting& Wang, Dan& Xia, Huanhuan& Zhao, Liangcai…[et al.]. High Glucose-Induced PC12 Cell Death by Increasing Glutamate Production and Decreasing Methyl Group Metabolism. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1097671
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097671