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Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina
Joint Authors
Lee, Hyun-Suk
Park, Kwangsung
Oh, Kyung-Jin
Ahn, Kyuyoun
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina.
Female Sprague-Dawley rats (230–240 g, n = 45 ) were divided into three groups and subjected to a sham operation (control group, n = 15 ), bilateral ovariectomy (Ovx group, n = 15 ), or bilateral ovariectomy followed by daily subcutaneous injection of 17β-estradiol (50 μg/kg/day, Ovx + Est group, n = 15 ).
The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot.
Results.
Expression of ZO-1 was diffuse in all groups, with the highest intensity in the superficial epithelium in the control group.
Occludin was localized in the intermediate and basal epithelium.
Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group.
Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement.
Conclusions.
Tight junctions are distinctly localized in rat vagina, and estrogen modulates the expression of tight junctions.
Further researches are needed to clarify the functional role of tight junctions in vaginal lubrication.
American Psychological Association (APA)
Oh, Kyung-Jin& Lee, Hyun-Suk& Ahn, Kyuyoun& Park, Kwangsung. 2016. Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097791
Modern Language Association (MLA)
Oh, Kyung-Jin…[et al.]. Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1097791
American Medical Association (AMA)
Oh, Kyung-Jin& Lee, Hyun-Suk& Ahn, Kyuyoun& Park, Kwangsung. Estrogen Modulates Expression of Tight Junction Proteins in Rat Vagina. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1097791
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097791