Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma
Joint Authors
Alabsi, Aied Mohammed
Muharram, Bushra
Lim, Kai Li
Paterson, Ian C.
Ali-Saeed, Rola
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-30
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Dracaena cinnabari Balf.f.
is a red resin endemic to Socotra Island, Yemen.
Although there have been several reports on its therapeutic properties, information on its cytotoxicity and anticancer effects is very limited.
This study utilized a bioassay-guided fractionation approach to determine the cytotoxic and apoptosis-inducing effects of D.
cinnabari on human oral squamous cell carcinoma (OSCC).
The cytotoxic effects of D.
cinnabari crude extract were observed in a panel of OSCC cell lines and were most pronounced in H400.
Only fractions DCc and DCd were active on H400 cells; subfractions DCc15 and DCd16 exhibited the greatest cytotoxicity against H400 cells and D.
cinnabari inhibited cells proliferation in a time-dependent manner.
This was achieved primarily via apoptosis where externalization of phospholipid phosphatidylserine was observed using DAPI/Annexin V fluorescence double staining mechanism studied through mitochondrial membrane potential assay cytochrome c enzyme-linked immunosorbent and caspases activities revealed depolarization of mitochondrial membrane potential (MMP) and significant activation of caspases 9 and 3/7, concomitant with S phase arrest.
Apoptotic proteins array suggested that MMP was regulated by Bcl-2 proteins family as results demonstrated an upregulation of Bax, Bad, and Bid as well as downregulation of Bcl-2.
Hence, D.
cinnabari has the potential to be developed as an anticancer agent.
American Psychological Association (APA)
Alabsi, Aied Mohammed& Lim, Kai Li& Paterson, Ian C.& Ali-Saeed, Rola& Muharram, Bushra. 2016. Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1097938
Modern Language Association (MLA)
Alabsi, Aied Mohammed…[et al.]. Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1097938
American Medical Association (AMA)
Alabsi, Aied Mohammed& Lim, Kai Li& Paterson, Ian C.& Ali-Saeed, Rola& Muharram, Bushra. Cell Cycle Arrest and Apoptosis Induction via Modulation of Mitochondrial Integrity by Bcl-2 Family Members and Caspase Dependence in Dracaena cinnabari-Treated H400 Human Oral Squamous Cell Carcinoma. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1097938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097938