A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation
Joint Authors
Dauben, Thomas Josef
Ziebart, Josefin
Bender, Thomas
Zaatreh, Sarah
Bader, Rainer
Kreikemeyer, B.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Electrical stimulation is a promising approach to enhance bone regeneration while having potential to inhibit bacterial growth.
To investigate effects of alternating electric field stimulation on both human osteoblasts and bacteria, a novel in vitro system was designed.
Electric field distribution was simulated numerically and proved by experimental validation.
Cells were stimulated on Ti6Al4V electrodes and in short distance to electrodes.
Bacterial growth was enumerated in supernatant and on the electrode surface and biofilm formation was quantified.
Electrical stimulation modulated gene expression of osteoblastic differentiation markers in a voltage-dependent manner, resulting in significantly enhanced osteocalcin mRNA synthesis rate on electrodes after stimulation with 1.4VRMS.
While collagen type I synthesis increased when stimulated with 0.2VRMS, it decreased after stimulation with 1.4VRMS.
Only slight and infrequent influence on bacterial growth was observed following stimulations with 0.2VRMS and 1.4VRMS after 48 and 72 h, respectively.
In summary this novel test system is applicable for extended in vitro studies concerning definition of appropriate stimulation parameters for bone cell growth and differentiation, bacterial growth suppression, and investigation of general effects of electrical stimulation.
American Psychological Association (APA)
Dauben, Thomas Josef& Ziebart, Josefin& Bender, Thomas& Zaatreh, Sarah& Kreikemeyer, B.& Bader, Rainer. 2016. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
Modern Language Association (MLA)
Dauben, Thomas Josef…[et al.]. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
American Medical Association (AMA)
Dauben, Thomas Josef& Ziebart, Josefin& Bender, Thomas& Zaatreh, Sarah& Kreikemeyer, B.& Bader, Rainer. A Novel In Vitro System for Comparative Analyses of Bone Cells and Bacteria under Electrical Stimulation. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1098020
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098020