Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data
Joint Authors
Silva, Artur
Ramos, Rommel
Kawasaki, Regiane
Baraúna, Rafael A.
Carepo, Marta S. P.
Oliveira, Rui
Marques, Rodolfo
Schneider, Maria P. C.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Exiguobacterium antarcticum B7 is extremophile Gram-positive bacteria able to survive in cold environments.
A key factor to understanding cold adaptation processes is related to the modification of fatty acids composing the cell membranes of psychrotrophic bacteria.
In our study we show the in silico reconstruction of the fatty acid biosynthesis pathway of E.
antarcticum B7.
To build the stoichiometric model, a semiautomatic procedure was applied, which integrates genome information using KEGG and RAST/SEED.
Constraint-based methods, namely, Flux Balance Analysis (FBA) and elementary modes (EM), were applied.
FBA was implemented in the sense of hexadecenoic acid production maximization.
To evaluate the influence of the gene expression in the fluxome analysis, FBA was also calculated using the log2FC values obtained in the transcriptome analysis at 0°C and 37°C.
The fatty acid biosynthesis pathway showed a total of 13 elementary flux modes, four of which showed routes for the production of hexadecenoic acid.
The reconstructed pathway demonstrated the capacity of E.
antarcticum B7 to de novo produce fatty acid molecules.
Under the influence of the transcriptome, the fluxome was altered, promoting the production of short-chain fatty acids.
The calculated models contribute to better understanding of the bacterial adaptation at cold environments.
American Psychological Association (APA)
Kawasaki, Regiane& Baraúna, Rafael A.& Silva, Artur& Carepo, Marta S. P.& Oliveira, Rui& Marques, Rodolfo…[et al.]. 2016. Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098871
Modern Language Association (MLA)
Kawasaki, Regiane…[et al.]. Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098871
American Medical Association (AMA)
Kawasaki, Regiane& Baraúna, Rafael A.& Silva, Artur& Carepo, Marta S. P.& Oliveira, Rui& Marques, Rodolfo…[et al.]. Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098871
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098871