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Potential Role of the Last Half Repeat in TAL Effectors Revealed by a Molecular Simulation Study
Joint Authors
Chang, Shan
Hu, Jian-ping
Wan, Hua
Tian, Xu-hong
Wang, Mei-hua
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-10
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
TAL effectors (TALEs) contain a modular DNA-binding domain that is composed of tandem repeats.
In all naturally occurring TALEs, the end of tandem repeats is invariantly a truncated half repeat.
To investigate the potential role of the last half repeat in TALEs, we performed comparative molecular dynamics simulations for the crystal structure of DNA-bound TALE AvrBs3 lacking the last half repeat and its modeled structure having the last half repeat.
The structural stability analysis indicates that the modeled system is more stable than the nonmodeled system.
Based on the principle component analysis, it is found that the AvrBs3 increases its structural compactness in the presence of the last half repeat.
The comparison of DNA groove parameters of the two systems implies that the last half repeat also causes the change of DNA major groove binding efficiency.
The following calculation of hydrogen bond reveals that, by stabilizing the phosphate binding with DNA at the C-terminus, the last half repeat helps to adopt a compact conformation at the protein-DNA interface.
It further mediates more contacts between TAL repeats and DNA nucleotide bases.
Finally, we suggest that the last half repeat is required for the high-efficient recognition of DNA by TALE.
American Psychological Association (APA)
Wan, Hua& Chang, Shan& Hu, Jian-ping& Tian, Xu-hong& Wang, Mei-hua. 2016. Potential Role of the Last Half Repeat in TAL Effectors Revealed by a Molecular Simulation Study. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098915
Modern Language Association (MLA)
Wan, Hua…[et al.]. Potential Role of the Last Half Repeat in TAL Effectors Revealed by a Molecular Simulation Study. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1098915
American Medical Association (AMA)
Wan, Hua& Chang, Shan& Hu, Jian-ping& Tian, Xu-hong& Wang, Mei-hua. Potential Role of the Last Half Repeat in TAL Effectors Revealed by a Molecular Simulation Study. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1098915
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098915