The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells
Joint Authors
Kong, Wei-Jia
Wang, Can
Jiang, Jian-Dong
Wu, Wei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-30
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The mangiferin-berberine (MB) salt was synthesized by ionic bonding of mangiferin (M) and berberine (B) at an equal molecular ratio.
This study aimed to investigate the activities of MB salt in modulating lipid and glucose metabolisms in HepG2 cells.
After 24 h treatment of the studying compounds, cellular AMP-activated protein kinase α (AMPKα)/acetyl-CoA carboxylase (ACC) protein levels and carnitine palmitoyltransferase (CPT) 1 activities, intracellular lipid contents, mRNA expression levels of target genes, glucose consumption, and glucose production amounts were determined.
Compound C (CC) was used in the blocking experiments.
Our results showed that MB salt increased p-AMPKα (Thr172)/p-ACC (Ser79) levels and CPT1 activity and suppressed oleic acid- (OA-) induced lipid accumulation and upregulation of lipogenic genes potently in HepG2 cells.
The above activities of MB salt were AMPK dependent and were superior to those of M or B when administered at an equal molar concentration.
MB salt enhanced basal and insulin-stimulated glucose consumption and suppressed gluconeogenesis more potently than M or B alone.
The inhibiting activity of MB salt on cellular gluconeogenesis was AMPK dependent.
Our results may support MB salt as a new kind of agent for the development of novel lipid or glucose-lowering drugs in the future.
American Psychological Association (APA)
Wang, Can& Jiang, Jian-Dong& Wu, Wei& Kong, Wei-Jia. 2016. The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1099134
Modern Language Association (MLA)
Wang, Can…[et al.]. The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells. BioMed Research International No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1099134
American Medical Association (AMA)
Wang, Can& Jiang, Jian-Dong& Wu, Wei& Kong, Wei-Jia. The Compound of Mangiferin-Berberine Salt Has Potent Activities in Modulating Lipid and Glucose Metabolisms in HepG2 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1099134
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099134