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Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity
Joint Authors
Takegawa, Daisuke
Yumoto, Hiromichi
Matsuo, Takashi
Hosokawa, Yuki
Hirao, Kouji
Nakanishi, Tadashi
Kitamura, Chiaki
Washio, Ayako
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis.
Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts.
In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA.
The signal transduction pathway activated with NOD-specific ligand was assessed by blocking assay with specific inhibitors and reporter assay.
In KN-3 cells, the expression level of NOD1 was stronger than that of NOD2 and the production of chemokines, such as CINC-1, CINC-2, CCL20, and MCP-1, was upregulated by stimulation with NOD1-specific ligand, but not with NOD2-specific ligand.
CINC-2 and CCL20 production by stimulation with NOD1-specific ligand was reduced by p38 MAPK and AP-1 signaling inhibitors.
Furthermore, the reporter assay demonstrated AP-1 activation in NOD1-specific ligand-stimulated KN-3 cells.
These findings indicated that NOD1 expressed in odontoblasts functions to upregulate the chemokines expression via p38-AP-1 signaling pathway and suggested that NOD1 may play important roles in the initiation and progression of pulpitis.
American Psychological Association (APA)
Hosokawa, Yuki& Hirao, Kouji& Yumoto, Hiromichi& Washio, Ayako& Nakanishi, Tadashi& Takegawa, Daisuke…[et al.]. 2016. Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity. BioMed Research International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1099279
Modern Language Association (MLA)
Hosokawa, Yuki…[et al.]. Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity. BioMed Research International No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1099279
American Medical Association (AMA)
Hosokawa, Yuki& Hirao, Kouji& Yumoto, Hiromichi& Washio, Ayako& Nakanishi, Tadashi& Takegawa, Daisuke…[et al.]. Functional Roles of NOD1 in Odontoblasts on Dental Pulp Innate Immunity. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1099279
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099279