![](/images/graphics-bg.png)
Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation
Joint Authors
Micera, Alessandra
Calder, Virginia L.
Di Zazzo, Antonio
Sgrulletta, Roberto
Bonini, Stefano
Esposito, Graziana
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Objective.
Vernal keratoconjunctivitis (VKC) is a chronic recurrent bilateral inflammation of the conjunctiva associated with atopy.
Several inflammatory and tissue remodeling factors contribute to VKC disease.
The aim is to provide a chip-based protein analysis in tears from patients suffering from quiescent or active VKC.
Methods.
This study cohort included 16 consecutive patients with VKC and 10 controls.
Participants were subjected to clinical assessment of ocular surface and tear sampling.
Total protein quantification, total protein sketch, and protein array (sixty protein candidates) were evaluated.
Results.
An overall increased Fluorescent Intensity expression was observed in VKC arrays.
Particularly, IL1β, IL15, IL21, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, ICAM2, βNGF, NT4, BDNF, βFGF, SCF, MMP1, and MMP2 were increased in quiescent VKC.
Of those candidates, only IL1β, IL15, IL21, βNGF, SCF, MMP2, Eotaxin2, TACE, MIP1α, MIP3α, NCAM1, and ICAM2 were increased in both active and quiescent VKC.
Finally, NT4, βFGF, and MMP1 were highly increased in active VKC.
Conclusion.
A distinct “protein tear-print” characterizes VKC activity, confirming some previously reported factors and highlighting some new candidates common to quiescent and active states.
Those candidates expressed in quiescent VKC might be considered as predictive indicators of VKC reactivation and/or exacerbation out-of-season.
American Psychological Association (APA)
Micera, Alessandra& Di Zazzo, Antonio& Esposito, Graziana& Sgrulletta, Roberto& Calder, Virginia L.& Bonini, Stefano. 2016. Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099342
Modern Language Association (MLA)
Micera, Alessandra…[et al.]. Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1099342
American Medical Association (AMA)
Micera, Alessandra& Di Zazzo, Antonio& Esposito, Graziana& Sgrulletta, Roberto& Calder, Virginia L.& Bonini, Stefano. Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1099342
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099342