Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications
Joint Authors
Safranow, Krzysztof
Urasinska, Elzbieta
Tarnowski, Maciej
Czerewaty, Michał
Deskur, Anna
Marlicz, Wojciech
Starzyńska, Teresa
Ratajczak, Mariusz Z.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background.
While cancer/testis antigens (CTAs) are restricted in postnatal tissues to testes and germ line-derived cells, their role in cancer development and the clinical significance of their expression still remain to be better defined.
Objective.
The aim of this study was to investigate the level of CTA expression in colon samples from patients with colorectal cancer (CRC) in relation to patient clinical status.
Methods.
Forty-five patients with newly diagnosed colorectal cancer were included in the study.
We selected a panel of 18 CTAs that were previously detected in CRC as well as some new gene candidates, and their expression was detected at the mRNA level by employing RQ-PCR.
Additionally, we evaluated CTA expression in three colon cancer cell lines (CL-188, HTB-39, and HTB-37) after exposure to the DNA methylation-modifying drug 5-azacytidine.
Results.
We report that 6 out of 18 (33%) CTAs tested (MAGEA3, OIP5, TTK, PLU1, DKKL1, and FBXO39) were significantly ( p < 0.05 ) overexpressed in tumor tissue compared with healthy colon samples isolated from the same patients.
Conclusions.
Moreover, we found that MAGEA3, PLU-1, and DKKL expression positively correlated with disease progression, evaluated according to the Dukes staging system.
Finally, 5-azacytidine exposure significantly upregulated expression of CTAs on CRC cells, which indicates that this demethylation agent could be employed therapeutically to enhance the immune response against tumor cells.
American Psychological Association (APA)
Tarnowski, Maciej& Czerewaty, Michał& Deskur, Anna& Safranow, Krzysztof& Marlicz, Wojciech& Urasinska, Elzbieta…[et al.]. 2016. Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications. Disease Markers،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103665
Modern Language Association (MLA)
Tarnowski, Maciej…[et al.]. Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications. Disease Markers No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1103665
American Medical Association (AMA)
Tarnowski, Maciej& Czerewaty, Michał& Deskur, Anna& Safranow, Krzysztof& Marlicz, Wojciech& Urasinska, Elzbieta…[et al.]. Expression of Cancer Testis Antigens in Colorectal Cancer: New Prognostic and Therapeutic Implications. Disease Markers. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103665
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103665