Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect
Joint Authors
Li, Jia
Lin, Xiaosheng
Zhang, Yuping
Liu, Weicai
Mi, Xiaohui
Zhang, Jiquan
Su, Jiansheng
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
To understand the material basis and underlying molecular machinery of antiosteoporosis activity of the Flos Chrysanthemi Indici (FCI), the consequences of ethanol extract on the bone loss in mice induced due to ovariectomy (OVX) was evaluated.
Also, the antiosteoporosis fraction obtained from the FCI ethanol extract was isolated and purified using a preparative high-speed countercurrent chromatography (HSCCC).
The in vitro impact of the compounds was investigated on osteoblast proliferation and differentiation.
The results revealed that ethyl acetate fraction with robust in vivo antiosteoporosis activity was obtained.
The important compounds purified by HSCCC using gradient elution system included acacetin, apigenin, luteolin, and linarin.
The four compounds enhanced the differentiation and proliferation of osteoblasts in MC3T3-E1 cells.
They also augmented the mRNA levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COL I).
The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds.
The present study demonstrated that the antiosteoporosis effects of FCI did not depend on a single component, and HSCCC efficiently isolated and purified the antiosteoporosis bioactive compounds from FCI.
American Psychological Association (APA)
Li, Jia& Lin, Xiaosheng& Zhang, Yuping& Liu, Weicai& Mi, Xiaohui& Zhang, Jiquan…[et al.]. 2016. Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103997
Modern Language Association (MLA)
Li, Jia…[et al.]. Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1103997
American Medical Association (AMA)
Li, Jia& Lin, Xiaosheng& Zhang, Yuping& Liu, Weicai& Mi, Xiaohui& Zhang, Jiquan…[et al.]. Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1103997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103997