Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells
Joint Authors
Xiong, Lingxin
Liu, Chuangui
Zhang, Xiaotian
Wang, Fang
Zhang, Chao
Sai, Jingying
Zheng, Jingtong
Lu, Yanjiao
Wang, Guoqiang
Wang, Yawei
Wang, Ting
Guan, Xuewa
Chen, Fang
Fang, Keyong
Lu, Junying
Zhu, Hailin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Yinhua Miyanling Tablet (YMT), the Chinese formula, has long been administrated in clinical practice for the treatment of acute pyelonephritis and acute urocystitis.
In the current study, we aimed to investigate the anti-inflammatory effect of YMT in vitro and to evaluate the association between anti-inflammation and innate immune response.
Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll density gradient centrifugation and then were stimulated by Lipopolysaccharide (LPS).
The differential gene expression of inflammation-related genes after drug administration was assessed using PCR array, and the protein levels of differential genes were measured by ELISA and Western blot.
The result showed that YMT significantly inhibited the expression of NLRP3, Caspase-1, and the downstream cytokine IL-1β and suppressed the production of inflammatory mediators TNF-α, IL-6, IL-10, and MCP-1 in a dose-dependent manner compared to the LPS group P < 0.01 .
The finding indicated that YMT exhibited anti-inflammatory effect in vitro by suppressing the NLRP3/Caspase-1 inflammasome, and that may have therapeutic potential for the treatment of inflammatory diseases.
American Psychological Association (APA)
Sai, Jingying& Xiong, Lingxin& Zheng, Jingtong& Liu, Chuangui& Lu, Yanjiao& Wang, Guoqiang…[et al.]. 2016. Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104017
Modern Language Association (MLA)
Sai, Jingying…[et al.]. Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1104017
American Medical Association (AMA)
Sai, Jingying& Xiong, Lingxin& Zheng, Jingtong& Liu, Chuangui& Lu, Yanjiao& Wang, Guoqiang…[et al.]. Protective Effect of Yinhua Miyanling Tablet on Lipopolysaccharide-Induced Inflammation through Suppression of NLRP3Caspase-1 Inflammasome in Human Peripheral Blood Mononuclear Cells. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1104017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104017