Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury
Joint Authors
Peng, Yuan
Tao, Yanyan
Liu, Chenghai
Zhao, Qiang
Huang, Kai
Lei, Yang
Liu, Hong-Liang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Salvianolate is widely used to treat angiocardiopathy in clinic in China, but its application in liver diseases remains unclear.
Our study aims to investigate the effect of Salvianolate on rat hepatic injury by protecting hepatocyte mitochondria.
To evaluate the effects of Salvianolate on injured hepatocytes, alpha mouse liver 12 (AML-12) cells were induced with hydrogen peroxide (H2O2) and treated with Salvianolate.
Cell viability and MitoTracker Green for mitochondria and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazole-carbocyanide iodine (JC-1) levels and cytochrome C (Cyto-C) expressions were detected in vitro.
To identify the effect of Salvianolate on protecting against mitochondria injury, male Wistar rats were injected with carbon tetrachloride (CCl4) and treated with Salvianolate (40 mg·kg−1).
Serum liver function, parameters for peroxidative damage, hematoxylin and eosin (H&E) staining, and transmission electron microscope (TEM) of hepatocyte mitochondria were assayed.
Our results showed that Salvianolate effectively protected hepatocytes, increased mitochondria vitality, and decreased Cyto-C expressions in vitro.
Besides, Salvianolate alleviated the liver function, attenuated the indicators of peroxidation, and relieved the mitochondria injury in vivo.
In conclusion, Salvianolate is effective in protecting hepatocytes from injury in vitro and in vivo, and the mechanism might be related to its protective effect on hepatocyte mitochondria against oxidative stress.
American Psychological Association (APA)
Zhao, Qiang& Peng, Yuan& Huang, Kai& Lei, Yang& Liu, Hong-Liang& Tao, Yanyan…[et al.]. 2016. Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104210
Modern Language Association (MLA)
Zhao, Qiang…[et al.]. Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1104210
American Medical Association (AMA)
Zhao, Qiang& Peng, Yuan& Huang, Kai& Lei, Yang& Liu, Hong-Liang& Tao, Yanyan…[et al.]. Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104210
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104210