Effects of Huang Qi Decoction on Endothelial Dysfunction Induced by Homocysteine
Joint Authors
Chu, Shuang
Mao, Xiao-dong
Wang, Li
Peng, Wen
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-20
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Vascular endothelial dysfunction can be induced by homocysteine (Hcy) through promoted oxidative stress.
Huang Qi decoction (HQD) is a traditional Chinese medical formula and its components possess antioxidant effect.
The study herein was therefore designed to investigate the effects of HQD at different dosage on endothelial dysfunction induced by Hcy.
Tempol and apocynin were used to investigate whether antioxidant mechanisms were involved.
Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings.
Reactive oxygen species (ROS) in human umbilical vein endothelial cells (HUVECs) was determined by DHE staining.
The assessment related to oxidative stress and NO bioavailability was performed by assay kits and western blot.
In isometric tension experiment, HQD at the dose of 30 or 100 μg/mL, tempol, or apocynin prevented impaired endothelium-dependent relaxation in isolated aortas elicited by Hcy.
In cellular experiments, substantial enhancement in NADPH oxidase and ROS generation and reduction in NO bioavailability triggered by Hcy were reversed by pretreatment of HQD at the dose of 100 μg/mL, tempol, or apocynin.
The results proved that HQD at an appropriate dosage presented favorable effects on endothelial dysfunction initiated by Hcy through antioxidant mechanisms.
HQD can act as a potent prescription for the treatment of endothelium related vascular complications.
American Psychological Association (APA)
Chu, Shuang& Mao, Xiao-dong& Wang, Li& Peng, Wen. 2016. Effects of Huang Qi Decoction on Endothelial Dysfunction Induced by Homocysteine. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104333
Modern Language Association (MLA)
Chu, Shuang…[et al.]. Effects of Huang Qi Decoction on Endothelial Dysfunction Induced by Homocysteine. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104333
American Medical Association (AMA)
Chu, Shuang& Mao, Xiao-dong& Wang, Li& Peng, Wen. Effects of Huang Qi Decoction on Endothelial Dysfunction Induced by Homocysteine. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104333
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104333