Identification of the Key Genes and Pathways in Esophageal Carcinoma
Joint Authors
Su, Peng
Wen, Shiwang
Zhang, Yuefeng
Li, Yong
Xu, Yanzhao
Zhu, Yonggang
Lv, Huilai
Zhang, Fan
Wang, Mingbo
Tian, Ziqiang
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Objective.
Esophageal carcinoma (EC) is a frequently common malignancy of gastrointestinal cancer in the world.
This study aims to screen key genes and pathways in EC and elucidate the mechanism of it.
Methods.
5 microarray datasets of EC were downloaded from Gene Expression Omnibus.
Differentially expressed genes (DEGs) were screened by bioinformatics analysis.
Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network construction were performed to obtain the biological roles of DEGs in EC.
Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression level of DEGs in EC.
Results.
A total of 1955 genes were filtered as DEGs in EC.
The upregulated genes were significantly enriched in cell cycle and the downregulated genes significantly enriched in Endocytosis.
PPI network displayed CDK4 and CCT3 were hub proteins in the network.
The expression level of 8 dysregulated DEGs including CDK4, CCT3, THSD4, SIM2, MYBL2, CENPF, CDCA3, and CDKN3 was validated in EC compared to adjacent nontumor tissues and the results were matched with the microarray analysis.
Conclusion.
The significantly DEGs including CDK4, CCT3, THSD4, and SIM2 may play key roles in tumorigenesis and development of EC involved in cell cycle and Endocytosis.
American Psychological Association (APA)
Su, Peng& Wen, Shiwang& Zhang, Yuefeng& Li, Yong& Xu, Yanzhao& Zhu, Yonggang…[et al.]. 2016. Identification of the Key Genes and Pathways in Esophageal Carcinoma. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104641
Modern Language Association (MLA)
Su, Peng…[et al.]. Identification of the Key Genes and Pathways in Esophageal Carcinoma. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1104641
American Medical Association (AMA)
Su, Peng& Wen, Shiwang& Zhang, Yuefeng& Li, Yong& Xu, Yanzhao& Zhu, Yonggang…[et al.]. Identification of the Key Genes and Pathways in Esophageal Carcinoma. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1104641
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104641