Deoxycholic Acid Could Induce Apoptosis and Trigger Gastric Carcinogenesis on Gastric Epithelial Cells by Quantitative Proteomic Analysis
Joint Authors
Zhang, Jing
Wang, Ye
Shi, Yanyan
Wei, Ying
Zhang, Ting
Ding, Shigang
Source
Gastroenterology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Pathologic duodenogastric reflux can induce or aggravate gastritis because of the presence of bile acids.
Bile reflux has been generally considered to be associated with intestinal metaplasia and gastric cancer.
However, the pathogenic mechanisms of the effects of bile acids on gastric mucosa are still unknown.
Methods.
To explore the mechanisms by which bile acids induce gastric mucosal lesions, we examined cell apoptosis in the gastric epithelial cell line GES-1 and investigated the changes in protein profiles of GES-1 cells in response to a bile acid deoxycholic acid using a proteomics approach.
Changes in the profiles of the differently expressed proteins were analyzed using the DAVID and STRING programs.
Results.
We found apoptosis was significantly induced in GES-1 cells by deoxycholic acid.
Using liquid chromatographic/tandem mass spectrometric (LC-MS/MS) methods, 134 upregulated proteins and 214 downregulated proteins were identified in the bile acid treated GES-1 cells.
Bioinformatics analysis revealed the interactions and signaling networks of these differentially expressed proteins.
Conclusion.
These findings may improve the understanding of the molecular mechanisms underlying the pathogenicity of bile acids on gastric mucosa.
American Psychological Association (APA)
Shi, Yanyan& Wei, Ying& Zhang, Ting& Zhang, Jing& Wang, Ye& Ding, Shigang. 2016. Deoxycholic Acid Could Induce Apoptosis and Trigger Gastric Carcinogenesis on Gastric Epithelial Cells by Quantitative Proteomic Analysis. Gastroenterology Research and Practice،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104846
Modern Language Association (MLA)
Shi, Yanyan…[et al.]. Deoxycholic Acid Could Induce Apoptosis and Trigger Gastric Carcinogenesis on Gastric Epithelial Cells by Quantitative Proteomic Analysis. Gastroenterology Research and Practice Vol. 2016, no. 2016 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1104846
American Medical Association (AMA)
Shi, Yanyan& Wei, Ying& Zhang, Ting& Zhang, Jing& Wang, Ye& Ding, Shigang. Deoxycholic Acid Could Induce Apoptosis and Trigger Gastric Carcinogenesis on Gastric Epithelial Cells by Quantitative Proteomic Analysis. Gastroenterology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1104846
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104846