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Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India
Joint Authors
Zaman, Shamsuz
Chaurasia, Rahul
Chatterjee, Kabita
Rout, Diptiranjan
Pandey, Hem Chandra
Maurya, Abhishek Kumar
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Introduction of nucleic acid testing (NAT) has helped in decreasing window period donations, resulting in increased safety of blood supplies.
NAT combines the advantages of direct and highly sequence-specific detection of viral genomes.
We analysed the performance of newer Procleix Ultrio Elite (PUE) and Procleix Ultrio assay (PUA) for the screening of the viral markers in our donor population.
Material and Methods.
10,015 donor samples were screened by routine immunoassays and both versions of NAT.
NAT yields detected were subjected to viral load estimation and to other serological markers.
Results.
A total of 21 NAT yields were detected; three were positive by both NAT systems, whereas 18 samples were reactive by PUE only.
NAT yields include 18 HBV and 3 HCV yields, of which 17 HBV yields were occult infections and 1 was window period (WP) infection.
All 3 HCV yields were WP infections.
No HIV-1/HIV-2 yield was found.
Conclusion.
Efficient target capture chemistry in the new TMA assay version significantly improved sensitivity.
NAT is superior to serological immunoassays for screening of the viral markers; and the efficient target capture system in the newer TMA assay, namely, the PUE system, has significantly improved sensitivity over the earlier versions.
American Psychological Association (APA)
Chaurasia, Rahul& Rout, Diptiranjan& Zaman, Shamsuz& Chatterjee, Kabita& Pandey, Hem Chandra& Maurya, Abhishek Kumar. 2016. Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106257
Modern Language Association (MLA)
Chaurasia, Rahul…[et al.]. Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India. International Journal of Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106257
American Medical Association (AMA)
Chaurasia, Rahul& Rout, Diptiranjan& Zaman, Shamsuz& Chatterjee, Kabita& Pandey, Hem Chandra& Maurya, Abhishek Kumar. Comparison of Procleix Ultrio Elite and Procleix Ultrio NAT Assays for Screening of Transfusion Transmitted Infections among Blood Donors in India. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106257
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106257