Diverse Molecular Genotypes of Mycobacterium tuberculosis Complex Isolates Circulating in the Free State, South Africa
Joint Authors
Van der Spoel van Dijk, Anneke
Makhoahle, Pakiso M.
Baba, Kamaldeen
Rigouts, Leen
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Tuberculosis is a serious public health concern especially in Africa and Asia.
Studies describing strain diversity are lacking in the Free State region of South Africa.
The aim of the study was to describe the diversity of Mycobacterium tuberculosis (M.
tuberculosis) strain families in the Free State province of South Africa.
A total of 86 M.
tuberculosis isolates were genotyped using spoligotyping.
A 12-locus mycobacterial interspersed repetitive units-variable-number tandem repeats (MIRU-VNTRs) typing was used to further characterize the resulting spoligotyping clusters.
SITVITWEB identified 49 different patterns with allocation to six lineages including Latin-American-Mediterranean (LAM) (18 isolates), T (14 isolates), Beijing (five isolates), S (six isolates), Haarlem (one isolate), and X (five isolates), while 37 (43.0%) orphans were identified.
Eight clusters included 37 isolates with identical spoligotypes (2 to 13/cluster).
MIRU-VNTR typing further differentiated three spoligotyping clusters: SIT1/Beijing/MIT17, SIT33/LAM3/MIT213, and confirmed one SIT34/S/MIT311.
In addition, SpolDB3/RIM assignment of the orphan strains resulted in a further 10 LAM and 13 T families.
In total, LAM (28 isolates) and T (27 isolates) cause 63% of the individual cases of MTB in our study.
The Free State has a highly diverse TB population with LAM being predominant.
Further studies with inclusion of multidrug-resistant strains with larger sample size are warranted.
American Psychological Association (APA)
Van der Spoel van Dijk, Anneke& Makhoahle, Pakiso M.& Rigouts, Leen& Baba, Kamaldeen. 2016. Diverse Molecular Genotypes of Mycobacterium tuberculosis Complex Isolates Circulating in the Free State, South Africa. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106280
Modern Language Association (MLA)
Van der Spoel van Dijk, Anneke…[et al.]. Diverse Molecular Genotypes of Mycobacterium tuberculosis Complex Isolates Circulating in the Free State, South Africa. International Journal of Microbiology No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1106280
American Medical Association (AMA)
Van der Spoel van Dijk, Anneke& Makhoahle, Pakiso M.& Rigouts, Leen& Baba, Kamaldeen. Diverse Molecular Genotypes of Mycobacterium tuberculosis Complex Isolates Circulating in the Free State, South Africa. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1106280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106280