Activity of Medicinal Plant Extracts on Multiplication of Mycobacterium tuberculosis under Reduced Oxygen Conditions Using Intracellular and Axenic Assays
Joint Authors
Gupta, Pooja D.
Bhatter, P.
Mistry, N.
Source
International Journal of Microbiology
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
Test the activity of selected medicinal plant extracts on multiplication of Mycobacterium tuberculosis under reduced oxygen concentration which represents nonreplicating conditions.
Material and Methods.
Acetone, ethanol and aqueous extracts of the plants Acorus calamus L.
(rhizome), Ocimum sanctum L.
(leaf), Piper nigrum L.
(seed), and Pueraria tuberosa DC.
(tuber) were tested on Mycobacterium tuberculosis H37Rv intracellularly using an epithelial cell (A549) infection model.
The extracts found to be active intracellularly were further studied axenically under reducing oxygen concentrations.
Results and Conclusions.
Intracellular multiplication was inhibited ≥60% by five of the twelve extracts.
Amongst these 5 extracts, in axenic culture, P.
nigrum (acetone) was active under aerobic, microaerophilic, and anaerobic conditions indicating presence of multiple components acting at different levels and P.
tuberosa (aqueous) showed bactericidal activity under microaerophilic and anaerobic conditions implying the influence of anaerobiosis on its efficacy.
P.
nigrum (aqueous) and A.
calamus (aqueous and ethanol) extracts were not active under axenic conditions but only inhibited intracellular growth of Mycobacterium tuberculosis, suggesting activation of host defense mechanisms to mediate bacterial killing rather than direct bactericidal activity.
American Psychological Association (APA)
Bhatter, P.& Gupta, Pooja D.& Mistry, N.. 2016. Activity of Medicinal Plant Extracts on Multiplication of Mycobacterium tuberculosis under Reduced Oxygen Conditions Using Intracellular and Axenic Assays. International Journal of Microbiology،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1106285
Modern Language Association (MLA)
Bhatter, P.…[et al.]. Activity of Medicinal Plant Extracts on Multiplication of Mycobacterium tuberculosis under Reduced Oxygen Conditions Using Intracellular and Axenic Assays. International Journal of Microbiology No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1106285
American Medical Association (AMA)
Bhatter, P.& Gupta, Pooja D.& Mistry, N.. Activity of Medicinal Plant Extracts on Multiplication of Mycobacterium tuberculosis under Reduced Oxygen Conditions Using Intracellular and Axenic Assays. International Journal of Microbiology. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1106285
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1106285