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Pepsin Digested Oat Bran Proteins: Separation, Antioxidant Activity, and Identification of New Peptides
Joint Authors
Vanvi, Ariane
Tsopmo, Apollinaire
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study was to determine pepsin hydrolysis conditions to produce digested oat bran proteins with higher radical scavenging activities and separate and identify peptides.
Isolated proteins were then digested with different concentrations of pepsin and incubation times.
Hydrolysates produced with 1 : 30 enzyme substrate (E/S) ratio and 2 h possessed the highest peroxyl radical scavenging activity, 608 ± 17 µM TE/g (compared to 456–474 µM TE/g for other digests), and was therefore subsequently fractionated into eight fractions (F1–F8) by high performance liquid chromatography (HPLC).
F1 and F2 had little activity because of their low protein contents.
Activities of F3–F8 were 447–874 µM TE/g, 20–36%, and 10–14% in the peroxyl, superoxide anion, and hydroxyl radical tests, respectively.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to identify a total of fifty peptides that may have contributed to the activity of F3, a fraction that better scavenged radicals.
American Psychological Association (APA)
Vanvi, Ariane& Tsopmo, Apollinaire. 2016. Pepsin Digested Oat Bran Proteins: Separation, Antioxidant Activity, and Identification of New Peptides. Journal of Chemistry،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107768
Modern Language Association (MLA)
Vanvi, Ariane& Tsopmo, Apollinaire. Pepsin Digested Oat Bran Proteins: Separation, Antioxidant Activity, and Identification of New Peptides. Journal of Chemistry No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1107768
American Medical Association (AMA)
Vanvi, Ariane& Tsopmo, Apollinaire. Pepsin Digested Oat Bran Proteins: Separation, Antioxidant Activity, and Identification of New Peptides. Journal of Chemistry. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1107768
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1107768