The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats
Joint Authors
Gao, Qin
Yu, Ying
Jia, Xian-Jie
Zhang, Wei-ping
Fang, Ting-ting
Hu, Jie
Ma, Shan-Feng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To investigate the effects of low dose ethanol feeding in diabetic rats and analyze its underlying mechanisms.
Methods.
Male Sprague-Dawley rats were divided into 4 groups: control (Con), diabetes at 4 weeks (DM4W), diabetes at 8 weeks (DM8W), and EtOH + DM8W.
After 8 weeks, hemodynamic parameters were recorded and heart weight/body weight (H/B) and hydroxyproline (Hp) content in myocardium were measured.
Morphology of collagen in myocardial tissue was observed with Masson’s trichrome staining method and collagen volume fraction (CVF) was analysed.
The mRNA expression of ALDH2 was assessed with Real-Time PCR.
The protein expressions of p-JNK and JNK were evaluated using western blot.
Results.
In contrast to Con group, there was no difference in hemodynamic parameters in DM4W group, but mean arterial pressure and heart rate were decreased in DM8W group, and the ratios of H/B, Hp, and CVF were markedly increased.
ALDH2 mRNA expression was decreased, while the ratio of p-JNK/JNK were increased.
Compared with DM8W group, the above indexes were improved in EtOH + DM8W group.
Conclusion.
With low dose ethanol intervention, enhanced ALDH2 expression can antagonize the happening of myocardial fibrosis in diabetic rats, which may be relevant with downregulating the JNK pathway.
American Psychological Association (APA)
Yu, Ying& Jia, Xian-Jie& Zhang, Wei-ping& Fang, Ting-ting& Hu, Jie& Ma, Shan-Feng…[et al.]. 2016. The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats. Journal of Diabetes Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1108082
Modern Language Association (MLA)
Yu, Ying…[et al.]. The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats. Journal of Diabetes Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1108082
American Medical Association (AMA)
Yu, Ying& Jia, Xian-Jie& Zhang, Wei-ping& Fang, Ting-ting& Hu, Jie& Ma, Shan-Feng…[et al.]. The Protective Effect of Low-Dose Ethanol on Myocardial Fibrosis through Downregulating the JNK Signaling Pathway in Diabetic Rats. Journal of Diabetes Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1108082
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108082