Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection
Joint Authors
Tipper, Donald J.
Szomolanyi-Tsuda, Eva
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-26
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Background.
U65, a self-aggregating peptide scaffold, traps fused protein antigens in yeast cells.
Conversion to Yeast Cell Particle (YCP) vaccines by partial removal of surface mannoproteins exposes β-glucan, mediating efficient uptake by antigen-presenting cells (APCs).
YCP vaccines are inexpensive, capable of rapid large-scale production and have potential for both parenteral and oral use.
Results.
YCP processing by alkaline hydrolysis exposes up to 20% of the glucan but converts scaffolded antigen and internal yeast proteins into a common aggregate, preventing selective yeast protein removal.
For U65-green fluorescent protein (GFP) or U65-Apolipoprotein A1 (ApoA1) subcutaneous vaccines, maximal IgG responses in mice required 10% glucan exposure.
IgG responses to yeast proteins were 5-fold lower.
Proteolytic mannoprotein removal produced YCPs with only 6% glucan exposure, insufficiently porous for selective removal of even native yeast proteins.
Vaccine efficacy was reduced 10-fold.
Current YCP formulations, therefore, are not suitable for human use but have considerable potential for use in feed animal vaccines.
Significantly, a YCP vaccine expressing a GFP fusion to VP1, the murine polyoma virus major capsid protein, after either oral or subcutaneous administration, protected mice against an intraperitoneal polyoma virus challenge, reducing viral DNA levels in spleen and liver by >98%.
American Psychological Association (APA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. 2016. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
Modern Language Association (MLA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
American Medical Association (AMA)
Tipper, Donald J.& Szomolanyi-Tsuda, Eva. Scaffolded Antigens in Yeast Cell Particle Vaccines Provide Protection against Systemic Polyoma Virus Infection. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1108742
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108742