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Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens
Joint Authors
Cras, Patrick
Lee, Wai-Ping
Goossens, Herman
Willekens, Barbara
Martínez-Cáceres, Eva
Cools, Nathalie
Berneman, Zwi N.
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-14
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
While emerging evidence indicates that dendritic cells (DC) play a central role in the pathogenesis of multiple sclerosis (MS), their modulation with immunoregulatory agents provides prospect as disease-modifying therapy.
Our observations reveal that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) treatment of monocyte-derived DC results in a semimature phenotype and anti-inflammatory cytokine profile as compared to conventional DC, in both healthy controls and MS patients.
Importantly, 1,25(OH)2D3-treated DC induce T cell hyporesponsiveness, as demonstrated in an allogeneic mixed leukocyte reaction.
Next, following a freeze-thaw cycle, 1,25(OH)2D3-treated immature DC could be recovered with a 78% yield and 75% viability.
Cryopreservation did not affect the expression of membrane markers by 1,25(OH)2D3-treated DC nor their capacity to induce T cell hyporesponsiveness.
In addition, the T cell hyporesponsiveness induced by 1,25(OH)2D3-treated DC is antigen-specific and robust since T cells retain their capacity to respond to an unrelated antigen and do not reactivate upon rechallenge with fully mature conventional DC, respectively.
These observations underline the clinical potential of tolerogenic DC (tolDC) to correct the immunological imbalance in MS.
Furthermore, the feasibility to cryopreserve highly potent tolDC will, ultimately, contribute to the large-scale production and the widely applicable use of tolDC.
American Psychological Association (APA)
Lee, Wai-Ping& Willekens, Barbara& Cras, Patrick& Goossens, Herman& Martínez-Cáceres, Eva& Berneman, Zwi N.…[et al.]. 2016. Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1108821
Modern Language Association (MLA)
Lee, Wai-Ping…[et al.]. Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens. Journal of Immunology Research No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1108821
American Medical Association (AMA)
Lee, Wai-Ping& Willekens, Barbara& Cras, Patrick& Goossens, Herman& Martínez-Cáceres, Eva& Berneman, Zwi N.…[et al.]. Immunomodulatory Effects of 1,25-Dihydroxyvitamin D3 on Dendritic Cells Promote Induction of T Cell Hyporesponsiveness to Myelin-Derived Antigens. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1108821
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108821