Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome
Joint Authors
Shao, Zonghong
Han, Yu
Wang, Huaquan
Source
Journal of Immunology Research
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
The myelodysplastic syndrome (MDS) comprises a group of clonal hematopoietic stem cell diseases characterized by cytopenia, dysplasia in one or more of the major myeloid lineages, ineffective hematopoiesis, and increased risk of development of acute myeloid leukemia (AML).
Macrophages are innate immune cells that ingest and degrade abnormal cells, debris, and foreign material and orchestrate inflammatory processes.
We analyzed the role of macrophages from MDS patients in vitro.
Methods.
Macrophages were induced from peripheral blood of patients with MDS via granulocyte macrophage colony-stimulating factor (GM-CSF).
Phagocytic capacity of macrophages was measured with carboxyfluorescein succinimidyl ester and fluorescent microspheres.
CD206 and signal regulatory protein alpha (SIRPα) on macrophages were detected by flow cytometry.
Inducible nitric oxide synthase (iNOS) was measured by ELISA method.
Results.
Compared with normal control group, the number of monocytes increased in MDS patients.
However, the monocytes showed impaired ability to induce macrophages and the number of macrophages induced from MDS samples was lower.
Further, we demonstrated that the ex vivo phagocytic function of macrophages from MDS patients was impaired and levels of reorganization receptors CD206 and SIRPα were lower.
Levels of iNOS secreted by macrophages in MDS were increased.
Conclusions.
Monocyte-derived macrophages are impaired in myelodysplastic syndromes.
American Psychological Association (APA)
Han, Yu& Wang, Huaquan& Shao, Zonghong. 2016. Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome. Journal of Immunology Research،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1108824
Modern Language Association (MLA)
Han, Yu…[et al.]. Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome. Journal of Immunology Research No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1108824
American Medical Association (AMA)
Han, Yu& Wang, Huaquan& Shao, Zonghong. Monocyte-Derived Macrophages Are Impaired in Myelodysplastic Syndrome. Journal of Immunology Research. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1108824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1108824