Redox-Responsive Nanophotosensitizer Composed of Chlorin e6-Conjugated Dextran for Photodynamic Treatment of Colon Cancer Cells
Joint Authors
Chu, Chong Woo
Ryu, Je Ho
Jeong, Young-IL
Lee, Hye Lim
Kim, Hyun Yul
Son, Gyung Mo
Kim, Hyung Wook
Kang, Dae-Hwan
Kwak, Tae Won
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-04
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
We synthesized dextran-chlorin e6 conjugates having disulfide linkage for specific targeting of colonic region and cancer cells.
Reductive end group of dextran was treated with sodium borocyanohydride and conjugated with cystamine.
Cystamine end group was conjugated with carboxylic acid of chlorin e6 (DEX6ss).
DEX6ss conjugates were formed as spherical nanoparticles with small sizes less than 100 nm.
Chlorin e6 (Ce6) was specifically released from DEX6ss nanoparticles in the presence of dextranase or glutathione (GSH), indicating that DEX6ss nanoparticles have responsiveness against dextranase and redox-environment.
In dark-toxicity test using normal cells and cancer cells, Ce6 and DEX6ss nanoparticles were practically nontoxic.
Intracellular delivery of DEX6ss nanoparticles was significantly improved compared to Ce6 itself.
DEX6ss nanoparticles achieved significantly higher ROS production and phototoxicity against HCT116 colon cancer cells than Ce6 itself.
Furthermore, DEX6ss nanoparticles showed enhanced tumor targeting efficiency and longer retention in the tumor tissues at in vivo animal study with HCT116 tumor-bearing mice.
Furthermore, DEX6ss nanoparticles have responsiveness against colonic enzyme, dextranase, indicating that they have potential of colon-specific delivery and dextranase-specific drug delivery capacity.
We fabricated colon-specific and tumor-targetable nanophotosensitizer using DEX6ss conjugates.
They showed improved cellular uptake ratio, phototoxicity, and colon-specificity.
We suggest that DEX6ss nanoparticles can be considered as a promising candidate for PDT of colon cancer.
American Psychological Association (APA)
Chu, Chong Woo& Ryu, Je Ho& Jeong, Young-IL& Kwak, Tae Won& Lee, Hye Lim& Kim, Hyun Yul…[et al.]. 2016. Redox-Responsive Nanophotosensitizer Composed of Chlorin e6-Conjugated Dextran for Photodynamic Treatment of Colon Cancer Cells. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1109153
Modern Language Association (MLA)
Chu, Chong Woo…[et al.]. Redox-Responsive Nanophotosensitizer Composed of Chlorin e6-Conjugated Dextran for Photodynamic Treatment of Colon Cancer Cells. Journal of Nanomaterials No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1109153
American Medical Association (AMA)
Chu, Chong Woo& Ryu, Je Ho& Jeong, Young-IL& Kwak, Tae Won& Lee, Hye Lim& Kim, Hyun Yul…[et al.]. Redox-Responsive Nanophotosensitizer Composed of Chlorin e6-Conjugated Dextran for Photodynamic Treatment of Colon Cancer Cells. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1109153
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109153