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An Innovative Cell Microincubator for Drug Discovery Based on 3D Silicon Structures
Joint Authors
Aredia, Francesca
Carpignano, Francesca
Surdo, Salvatore
Barillaro, Giuseppe
Mazzini, Giuliano
Merlo, Sabina
Scovassi, Anna Ivana
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
We recently employed three-dimensional (3D) silicon microstructures (SMSs) consisting in arrays of 3 μm-thick silicon walls separated by 50 μm-deep, 5 μm-wide gaps, as microincubators for monitoring the biomechanical properties of tumor cells.
They were here applied to investigate the in vitro behavior of HT1080 human fibrosarcoma cells driven to apoptosis by the chemotherapeutic drug Bleomycin.
Our results, obtained by fluorescence microscopy, demonstrated that HT1080 cells exhibited a great ability to colonize the narrow gaps.
Remarkably, HT1080 cells grown on 3D-SMS, when treated with the DNA damaging agent Bleomycin under conditions leading to apoptosis, tended to shrink, reducing their volume and mimicking the normal behavior of apoptotic cells, and were prone to leave the gaps.
Finally, we performed label-free detection of cells adherent to the vertical silicon wall, inside the gap of 3D-SMS, by exploiting optical low coherence reflectometry using infrared, low power radiation.
This kind of approach may become a new tool for increasing automation in the drug discovery area.
Our results open new perspectives in view of future applications of the 3D-SMS as the core element of a lab-on-a-chip suitable for screening the effect of new molecules potentially able to kill tumor cells.
American Psychological Association (APA)
Aredia, Francesca& Carpignano, Francesca& Surdo, Salvatore& Barillaro, Giuseppe& Mazzini, Giuliano& Scovassi, Anna Ivana…[et al.]. 2016. An Innovative Cell Microincubator for Drug Discovery Based on 3D Silicon Structures. Journal of Nanomaterials،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109411
Modern Language Association (MLA)
Aredia, Francesca…[et al.]. An Innovative Cell Microincubator for Drug Discovery Based on 3D Silicon Structures. Journal of Nanomaterials No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1109411
American Medical Association (AMA)
Aredia, Francesca& Carpignano, Francesca& Surdo, Salvatore& Barillaro, Giuseppe& Mazzini, Giuliano& Scovassi, Anna Ivana…[et al.]. An Innovative Cell Microincubator for Drug Discovery Based on 3D Silicon Structures. Journal of Nanomaterials. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1109411
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1109411