Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice
Joint Authors
Jørgensen, L. H.
Ellman, Ditte Gry
Degn, Matilda
Lund, Minna Christiansen
Clausen, Bettina Hjelm
Novrup, Hans Gram
Flæng, Simon Bertram
Suntharalingam, Lujitha
Svenningsen, Åsa Fex
Brambilla, Roberta
Lambertsen, Kate Lykke
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-14
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Traumatic spinal cord injury (SCI) is followed by an instant increase in expression of the microglial-derived proinflammatory cytokine tumor necrosis factor (TNF) within the lesioned cord.
TNF exists both as membrane-anchored TNF (mTNF) and as cleaved soluble TNF (solTNF).
We previously demonstrated that epidural administration of a dominant-negative inhibitor of solTNF, XPro1595, to the contused spinal cord resulted in changes in Iba1 protein expression in microglia/macrophages, decreased lesion volume, and improved locomotor function.
Here, we extend our studies using mice expressing mTNF, but no solTNF ( m T N F Δ / Δ ), to study the effect of genetic ablation of solTNF on SCI.
We demonstrate that TNF levels were significantly decreased within the lesioned spinal cord 3 days after SCI in m T N F Δ / Δ mice compared to littermates.
This decrease did, however, not translate into significant changes in other pro- and anti-inflammatory cytokines (IL-10, IL-1β, IL-6, IL-5, IL-2, CXCL1, CCL2, or CCL5), despite a tendency towards increased IL-10 and decreased IL-1β, TNFR1, and TNFR2 levels in m T N F Δ / Δ mice.
In addition, microglial and leukocyte infiltration, activation state (Iba1, CD11b, CD11c, CD45, and MHCII), lesion size, and functional outcome after moderate SCI were comparable between genotypes.
Collectively, our data demonstrate that genetic ablation of solTNF does not significantly modulate postlesion outcome after SCI.
American Psychological Association (APA)
Ellman, Ditte Gry& Degn, Matilda& Lund, Minna Christiansen& Clausen, Bettina Hjelm& Novrup, Hans Gram& Flæng, Simon Bertram…[et al.]. 2016. Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1110974
Modern Language Association (MLA)
Ellman, Ditte Gry…[et al.]. Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice. Mediators of Inflammation No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1110974
American Medical Association (AMA)
Ellman, Ditte Gry& Degn, Matilda& Lund, Minna Christiansen& Clausen, Bettina Hjelm& Novrup, Hans Gram& Flæng, Simon Bertram…[et al.]. Genetic Ablation of Soluble TNF Does Not Affect Lesion Size and Functional Recovery after Moderate Spinal Cord Injury in Mice. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1110974
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110974