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GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway
Joint Authors
Wang, Yajing
Lu, Ping
Zhang, Weifeng
Du, Qianming
Tang, Jingjing
Wang, Hong
Lu, Jinrong
Hu, Rong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-23
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Nonresolving inflammation is one of the consistent features of the tumor microenvironment in the intestine and plays a critical role in the initiation and development of colon cancer.
Here we reported the inhibitory effects of GEN-27, a new derivative of genistein, on the inflammation-related colon cancer cell proliferation and delineated the mechanism of its action.
The results indicated that GEN-27 inhibited the proliferation of human colon tumor HCT116 cells stimulated by culture supernatants of LPS-induced human monocytes THP-1 cells and significantly decreased LPS-induced secretion of proinflammatory cytokines interleukin-6 and interleukin-1β in THP-1 cells.
The HCT116 cell proliferation elicited by THP-1-conditioned medium could be blocked by the interleukin-1 receptor antagonist (IL-1RA).
Further mechanistic study revealed that GEN-27 remarkably inhibited the nuclear translocation of NF- κ B and phosphorylation of I κ B and IKK α / β in both HCT116 and THP-1 cells.
In addition, GEN-27 markedly suppressed the HCT116 cell proliferation stimulated by IL-1β treatment, which was dependent on the inhibition of NF-κB/p65 nuclear localization, as verified by p65 overexpression and BAY 11-7082, an NF- κ B inhibitor.
Taken together, our findings established that GEN-27 modulated NF- κ B signaling pathway involved in inflammation-induced cancer cells proliferation and therefore could be a potential chemopreventive agent against inflammation-associated colon cancer.
American Psychological Association (APA)
Wang, Yajing& Lu, Ping& Zhang, Weifeng& Du, Qianming& Tang, Jingjing& Wang, Hong…[et al.]. 2016. GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1110981
Modern Language Association (MLA)
Wang, Yajing…[et al.]. GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway. Mediators of Inflammation No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1110981
American Medical Association (AMA)
Wang, Yajing& Lu, Ping& Zhang, Weifeng& Du, Qianming& Tang, Jingjing& Wang, Hong…[et al.]. GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1110981
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1110981