Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells
Joint Authors
Elfeky, Mohamed
Kaede, Ryuji
Okamatsu-Ogura, Yuko
Kimura, Kazuhiro
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
High mobility group protein B1 (HMGB1) is a late inflammatory mediator that exaggerates septic symptoms.
Adiponectin, an adipokine, has potent anti-inflammatory properties.
However, possible effects of adiponectin on lipopolysaccharide- (LPS-) induced HMGB1 release are unknown.
The aim of this study was to investigate effects of full length adiponectin on HMGB1 release in LPS-stimulated RAW 264 macrophage cells.
Treatment of the cells with LPS alone significantly induced HMGB1 release associated with HMGB1 translocation from the nucleus to the cytosol.
However, prior treatment with adiponectin suppressed LPS-induced HMGB1 release and translocation.
The anti-inflammatory cytokine interleukin- (IL-) 10 similarly suppressed LPS-induced HMGB1 release.
Adiponectin treatment decreased toll-like receptor 4 (TLR4) mRNA expression and increased heme oxygenase- (HO-) 1 mRNA expression without inducing IL-10 mRNA, while IL-10 treatment decreased TLR2 and HMGB1 mRNA expression and increased the expression of IL-10 and HO-1 mRNA.
Treatment with the HO-1 inhibitor ZnPP completely prevented the suppression of HMGB1 release by adiponectin but only partially inhibited that induced by IL-10.
Treatment with compound C, an AMP kinase (AMPK) inhibitor, abolished the increase in HO-1 expression and the suppression of HMGB1 release mediated by adiponectin.
In conclusion, our results indicate that adiponectin suppresses HMGB1 release by LPS through an AMPK-mediated and HO-1-dependent IL-10-independent pathway.
American Psychological Association (APA)
Elfeky, Mohamed& Kaede, Ryuji& Okamatsu-Ogura, Yuko& Kimura, Kazuhiro. 2016. Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells. Mediators of Inflammation،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111116
Modern Language Association (MLA)
Elfeky, Mohamed…[et al.]. Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells. Mediators of Inflammation No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1111116
American Medical Association (AMA)
Elfeky, Mohamed& Kaede, Ryuji& Okamatsu-Ogura, Yuko& Kimura, Kazuhiro. Adiponectin Inhibits LPS-Induced HMGB1 Release through an AMP Kinase and Heme Oxygenase-1-Dependent Pathway in RAW 264 Macrophage Cells. Mediators of Inflammation. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1111116
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1111116