Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia
Joint Authors
Yao, Chen
Li, Gang
Qian, Yeyong
Cai, Ming
Yin, Hong
Xiao, Li
Tang, Wei
Guo, Fengjie
Shi, Bingyi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
To investigate the effect of pentoxifylline (PTX) on spermatogenesis dysfunction induced by intermittent hypobaric hypoxia (IHH) and unveil the underlying mechanism, experimental animals were assigned to Control, IHH+Vehicle, and IHH+PTX groups and exposed to 4 cycles of 96 h of hypobaric hypoxia followed by 96 h of normobaric normoxia for 32 days.
PTX was administered for 32 days.
Blood and tissue samples were collected 7 days thereafter.
Serum malondialdehyde levels were used to assess lipid peroxidation; ferric-reducing antioxidant power (FRAP), superoxide dismutase, and catalase and glutathione peroxidase enzyme activities were assessed to determine antioxidant capacity in various samples.
Testis histopathology was assessed after hematoxylin-eosin staining by Johnsen’s testicular scoring system.
Meanwhile, testosterone synthase and vimentin amounts were assessed by immunohistochemistry.
Sperm count, motility, and density were assessed to determine epididymal sperm quality.
IHH treatment induced significant pathological changes in testicular tissue and enhanced serum lipid peroxide levels, while reducing serum FRAP, antioxidant enzyme activities, and testosterone synthase expression.
Moreover, IHH impaired epididymal sperm quality and vimentin structure in Sertoli cells.
Oral administration of PTX improved the pathological changes in the testis.
IHH may impair spermatogenesis function of testicular tissues by inducing oxidative stress, but this impairment could be attenuated by administration of PTX.
American Psychological Association (APA)
Yao, Chen& Li, Gang& Qian, Yeyong& Cai, Ming& Yin, Hong& Xiao, Li…[et al.]. 2016. Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113808
Modern Language Association (MLA)
Yao, Chen…[et al.]. Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113808
American Medical Association (AMA)
Yao, Chen& Li, Gang& Qian, Yeyong& Cai, Ming& Yin, Hong& Xiao, Li…[et al.]. Protection of Pentoxifylline against Testis Injury Induced by Intermittent Hypobaric Hypoxia. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113808
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113808