Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation
Joint Authors
Yao, Weifeng
Chi, Xinjin
Zhang, Yihan
Yuan, Dongdong
Zhu, Qianqian
Liu, Yue
Huang, Fei
Feng, Jiayu
Chen, Xi
Huang, Yong
Hei, Ziqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-31
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Aim.
To investigate whether hyperglycemia will aggravate hepatic ischemia reperfusion injury (HIRI) and the underlying mechanisms.
Methods.
Control and streptozotocin-induced diabetic Sprague-Dawley rats were subjected to partial hepatic ischemia reperfusion.
Liver histology, transferase, inflammatory cytokines, and oxidative stress were assessed accordingly.
Similarly, BRL-3A hepatocytes were subjected to hypoxia/reoxygenation (H/R) after high (25 mM) or low (5.5 mM) glucose culture.
Cell viability, reactive oxygen species (ROS), and activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and nuclear factor of kappa light polypeptide gene enhancer in B-cells (NF-κB) were determined.
Results.
Compared with control, diabetic rats presented more severe hepatic injury and increased hepatic inflammatory cytokines and oxidative stress.
HIRI in diabetic rats could be ameliorated by pretreatment of N-acetyl-L-cysteine (NAC) or apocynin.
Excessive ROS generation and consequent Nrf2 and NF-κB translocation were determined after high glucose exposure.
NF-κB translocation and its downstream cytokines were further increased in high glucose cultured group after H/R.
While proper regulation of Nrf2 to its downstream antioxidases was observed in low glucose cultured group, no further induction of Nrf2 pathway by H/R after high glucose culture was identified.
Conclusion.
Hyperglycemia aggravates HIRI, which might be attributed to chronic oxidative stress and inflammation and potential malfunction of antioxidative system.
American Psychological Association (APA)
Zhang, Yihan& Yuan, Dongdong& Yao, Weifeng& Zhu, Qianqian& Liu, Yue& Huang, Fei…[et al.]. 2016. Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1113870
Modern Language Association (MLA)
Zhang, Yihan…[et al.]. Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1113870
American Medical Association (AMA)
Zhang, Yihan& Yuan, Dongdong& Yao, Weifeng& Zhu, Qianqian& Liu, Yue& Huang, Fei…[et al.]. Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1113870
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113870