Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2STAT3 Signaling
Joint Authors
Han, Shichao
Cai, Weixia
Yang, Xuekang
Jia, Yanhui
Zheng, Zhao
Wang, Hongtao
Gao, Jianxin
Hu, Dahai
Yang, Tao
Guan, Hao
Zhu, Xiongxiang
Guo, Haitao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Oxidative stress plays an important role in burn-induced myocardial injury, but the cellular mechanisms that control reactive oxygen species (ROS) production and scavenging are not fully understood.
This study demonstrated that blockade of Notch signaling via knockout of the transcription factor RBP-J or a pharmacological inhibitor aggravated postburn myocardial injury, which manifested as deteriorated serum CK, CK-MB, and LDH levels and increased apoptosis in vitro and in vivo.
Interruption of Notch signaling increased intracellular ROS production, and a ROS scavenger reversed the exacerbated myocardial injury after Notch signaling blockade.
These results suggest that Notch signaling deficiency aggravated postburn myocardial injury through increased ROS levels.
Notch signaling blockade also decreased MnSOD expression in vitro and in vivo.
Notably, Notch signaling blockade downregulated p-JAK2 and p-STAT3 expression.
Inhibition of JAK2/STAT3 signaling with AG490 markedly decreased MnSOD expression, increased ROS production, and aggravated myocardial injury.
AG490 plus GSI exerted no additional effects.
These results demonstrate that Notch signaling protects against burn-induced myocardial injury through JAK2/STAT3 signaling, which activates the expression of MnSOD and leads to decreased ROS levels.
American Psychological Association (APA)
Cai, Weixia& Yang, Xuekang& Han, Shichao& Guo, Haitao& Zheng, Zhao& Wang, Hongtao…[et al.]. 2016. Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2STAT3 Signaling. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114032
Modern Language Association (MLA)
Cai, Weixia…[et al.]. Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2STAT3 Signaling. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1114032
American Medical Association (AMA)
Cai, Weixia& Yang, Xuekang& Han, Shichao& Guo, Haitao& Zheng, Zhao& Wang, Hongtao…[et al.]. Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2STAT3 Signaling. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114032
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114032