![](/images/graphics-bg.png)
Identification of Redox and Glucose-Dependent Txnip Protein Interactions
Joint Authors
Forred, Benjamin J.
Neuharth, Skyla
Kim, Dae In
Amolins, Michael W.
Motamedchaboki, Khatereh
Roux, Kyle J.
Vitiello, Peter F.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-29
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Thioredoxin-interacting protein (Txnip) acts as a negative regulator of thioredoxin function and is a critical modulator of several diseases including, but not limited to, diabetes, ischemia-reperfusion cardiac injury, and carcinogenesis.
Therefore, Txnip has become an attractive therapeutic target to alleviate disease pathologies.
Although Txnip has been implicated with numerous cellular processes such as proliferation, fatty acid and glucose metabolism, inflammation, and apoptosis, the molecular mechanisms underlying these processes are largely unknown.
The objective of these studies was to identify Txnip interacting proteins using the proximity-based labeling method, BioID, to understand differential regulation of pleiotropic Txnip cellular functions.
The BioID transgene fused to Txnip expressed in HEK293 identified 31 interacting proteins.
Many protein interactions were redox-dependent and were disrupted through mutation of a previously described reactive cysteine (C247S).
Furthermore, we demonstrate that this model can be used to identify dynamic Txnip interactions due to known physiological regulators such as hyperglycemia.
These data identify novel Txnip protein interactions and demonstrate dynamic interactions dependent on redox and glucose perturbations, providing clarification to the pleiotropic cellular functions of Txnip.
American Psychological Association (APA)
Forred, Benjamin J.& Neuharth, Skyla& Kim, Dae In& Amolins, Michael W.& Motamedchaboki, Khatereh& Roux, Kyle J.…[et al.]. 2016. Identification of Redox and Glucose-Dependent Txnip Protein Interactions. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114055
Modern Language Association (MLA)
Forred, Benjamin J.…[et al.]. Identification of Redox and Glucose-Dependent Txnip Protein Interactions. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114055
American Medical Association (AMA)
Forred, Benjamin J.& Neuharth, Skyla& Kim, Dae In& Amolins, Michael W.& Motamedchaboki, Khatereh& Roux, Kyle J.…[et al.]. Identification of Redox and Glucose-Dependent Txnip Protein Interactions. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114055