Induction Effect of Bisphenol A on Gene Expression Involving Hepatic Oxidative Stress in Rat

Abstract EN

Background and Objective.

Bisphenol A (BPA) is an abundantly used xenoestrogenic chemical which may cause various disorders in body.

In the present study, we sought to investigate the effects of various doses of BPA on hepatic oxidative stress-related gene expression in rats.

Methods.

Male Wistar rats weighing 150–200 g were used in this study.

Three doses of the BPA (5, 25, and 125 μg/kg) in corn oil were administered as gavage during 35 consecutive days.

After the experiment, the rats were expired and the livers were removed and stored at −80°C freezer for RNA extraction.

Findings.

The Real Time PCR showed increased expression of HO-1 in the rats receiving BPA doses compared to the control group.

This effect was dose-dependent and higher at doses of 25 and 125 μg/kg than 5 μg/kg of body weight (p<0.05).

It was also demonstrated that various doses BPA can increase GADD45B gene expression compared to control group.

That expression was significantly dominant in the lowest dose (5 μg/kg) of the BPA (p<0.05).

The final body weights (168.0±10.0 gr) in the treatment group [BPA (125 μg/kg)] showed a significant decrease compared to control group (191.60±6.50 gr).

Conclusion.

These findings demonstrate that BPA generated ROS and increased the antioxidant gene expression that causes hepatotoxicity.