Differential Expression of NADPH Oxidases Depends on Skeletal Muscle Fiber Type in Rats
Joint Authors
Loureiro, Adriano César Carneiro
Rêgo-Monteiro, Igor Coutinho do
Louzada, Ruy A.
Ortenzi, Victor Hugo
Aguiar, Angélica Ponte de
Abreu, Ewerton Sousa de
Cavalcanti-de-Albuquerque, João Paulo Albuquerque
Hecht, Fabio
Oliveira, Ariclécio Cunha de
Ceccatto, Vânia Marilande
Fortunato, Rodrigo S.
Carvalho, Denise P.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
NADPH oxidases (NOX) are important sources of reactive oxygen species (ROS) in skeletal muscle, being involved in excitation-contraction coupling.
Thus, we aimed to investigate if NOX activity and expression in skeletal muscle are fiber type specific and the possible contribution of this difference to cellular oxidative stress.
Oxygen consumption rate, NOX activity and mRNA levels, and the activity of catalase (CAT), glutathione peroxidase (GPX), and superoxide dismutase (SOD), as well as the reactive protein thiol levels, were measured in the soleus (SOL), red gastrocnemius (RG), and white gastrocnemius (WG) muscles of rats.
RG showed higher oxygen consumption flow than SOL and WG, while SOL had higher oxygen consumption than WG.
SOL showed higher NOX activity, as well as NOX2 and NOX4 mRNA levels, antioxidant enzymatic activities, and reactive protein thiol contents when compared to WG and RG.
NOX activity and NOX4 mRNA levels as well as antioxidant enzymatic activities were higher in RG than in WG.
Physical exercise increased NOX activity in SOL and RG, specifically NOX2 mRNA levels in RG and NOX4 mRNA levels in SOL.
In conclusion, we demonstrated that NOX activity and expression differ according to the skeletal muscle fiber type, as well as antioxidant defense.
American Psychological Association (APA)
Loureiro, Adriano César Carneiro& Rêgo-Monteiro, Igor Coutinho do& Louzada, Ruy A.& Ortenzi, Victor Hugo& Aguiar, Angélica Ponte de& Abreu, Ewerton Sousa de…[et al.]. 2016. Differential Expression of NADPH Oxidases Depends on Skeletal Muscle Fiber Type in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114132
Modern Language Association (MLA)
Loureiro, Adriano César Carneiro…[et al.]. Differential Expression of NADPH Oxidases Depends on Skeletal Muscle Fiber Type in Rats. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114132
American Medical Association (AMA)
Loureiro, Adriano César Carneiro& Rêgo-Monteiro, Igor Coutinho do& Louzada, Ruy A.& Ortenzi, Victor Hugo& Aguiar, Angélica Ponte de& Abreu, Ewerton Sousa de…[et al.]. Differential Expression of NADPH Oxidases Depends on Skeletal Muscle Fiber Type in Rats. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114132
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114132