Ginkgo Biloba Ameliorates Subfertility Induced by Testicular IschemiaReperfusion Injury in Adult Wistar Rats: A Possible New Mitochondrial Mechanism

Abstract EN

Testicular torsion, a surgical emergency, could affect the endocrine and exocrine testicular functions.

This study demonstrates histopathological and physiological effects of testicular ischemia/perfusion (I/R) injury and the possible protective effects of Ginkgo biloba treatment.

Fifty adult male Wistar rats, 180–200 gm, were randomly divided into sham-operated, Gingko biloba supplemented, ischemia only, I/R, and Gingko biloba treated I/R groups.

Overnight fasted rats were anaesthetized by Pentobarbital; I/R was performed by left testis 720° rotation in I/R and treated I/R groups.

Orchiectomy was performed for histopathological studies and detection of mitochondrial NAD+.

Determination of free testosterone, FSH, TNF-α, and IL1-β in plasma was performed.

Plasma-free testosterone was significantly decreased, while plasma FSH, TNF-α, IL-1β, and testicular mitochondrial NAD+ were significantly increased in I/R group compared to control group.

These parameters were reversed in Gingko biloba treated I/R group compared to I/R group.

I/R caused marked testicular damage and increased APAF-1 in the apoptotic cells which were reversed by Ginkgo biloba treatment.

It could be concluded that I/R caused subfertility induced by apoptosis and oxidative stress manifested by the elevated testicular mitochondrial NAD+, which is considered a new possible mechanism.

Also, testicular injury could be reduced by Gingko biloba administration alone.