Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2
Joint Authors
Zhang, Chun-yan
Tian, Ya-Ping
Zhang, Pengjun
Luo, Yuan
Wen, Xinyu
Wang, Ling
Gao, Jing
Wang, Zi
Lu, Chengrong
Duan, Lianning
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Although both oxidative stress and microRNAs (miRNAs) play vital roles in physiological and pathological processes, little is known about the interactions between them.
In this study, we first described the regulation of H2O2 in cell viability, proliferation, cycle, and apoptosis of human hepatocellular carcinoma cell line HepG2.
Then, miRNAs expression was profiled after H2O2 treatment.
The results showed that high concentration of H2O2 (600 μM) could decrease cell viability, inhibit cell proliferation, induce cell cycle arrest, and finally promote cell apoptosis.
Conversely, no significant effects could be found under treatment with low concentration (30 μM).
miRNAs array analysis identified 131 differentially expressed miRNAs (125 were upregulated and 6 were downregulated) and predicted 13504 putative target genes of the deregulated miRNAs.
Gene ontology (GO) analysis revealed that the putative target genes were associated with H2O2-induced cell growth arrest and apoptosis.
The subsequent bioinformatics analysis indicated that H2O2-response pathways, including MAPK signaling pathway, apoptosis, and pathways in cancer and cell cycle, were significantly affected.
Overall, these results provided comprehensive information on the biological function of H2O2 treatment in HepG2 cells.
The identification of miRNAs and their putative targets may offer new diagnostic and therapeutic strategies for liver cancer.
American Psychological Association (APA)
Luo, Yuan& Wen, Xinyu& Wang, Ling& Gao, Jing& Wang, Zi& Zhang, Chun-yan…[et al.]. 2016. Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114209
Modern Language Association (MLA)
Luo, Yuan…[et al.]. Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1114209
American Medical Association (AMA)
Luo, Yuan& Wen, Xinyu& Wang, Ling& Gao, Jing& Wang, Zi& Zhang, Chun-yan…[et al.]. Identification of MicroRNAs Involved in Growth Arrest and Apoptosis in Hydrogen Peroxide-Treated Human Hepatocellular Carcinoma Cell Line HepG2. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114209