Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes
Joint Authors
Martínez Martínez, Alejandro
Barraza-Garza, Guillermo
Castillo-Michel, Hiram
de la Rosa, Laura A.
Pérez-León, Jorge A.
Cotte, Marine
Alvarez-Parrilla, Emilio
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The protective effect of different polyphenols, catechin (Cat), quercetin (Qc) (flavonoids), gallic acid (GA), caffeic acid (CfA), chlorogenic acid (ChA) (phenolic acids), and capsaicin (Cap), against H2O2-induced oxidative stress was evaluated in rat enterocytes using Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) Spectroscopy and Fourier Transform Infrared Microspectroscopy (FTIRM), and results were compared to standard lipid peroxidation techniques: conjugated dienes (CD) and Thiobarbituric Acid Reactive Substances (TBARS).
Analysis of ATR-FTIR and FTIRM spectral data allowed the simultaneous evaluation of the effects of H2O2 and polyphenols on lipid and protein oxidation.
All polyphenols showed a protective effect against H2O2-induced oxidative stress in enterocytes, when administered before or after H2O2.
Cat and capsaicin showed the highest protective effect, while phenolic acids had weaker effects and Qc presented a mild prooxidative effect (IR spectral profile of biomolecules between control and H2O2-treated cells) according to FTIR analyses.
These results demonstrated the viability to use infrared spectroscopy to evaluate the oxidant and antioxidant effect of molecules in cell systems assays.
American Psychological Association (APA)
Barraza-Garza, Guillermo& Castillo-Michel, Hiram& de la Rosa, Laura A.& Martínez Martínez, Alejandro& Pérez-León, Jorge A.& Cotte, Marine…[et al.]. 2016. Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114731
Modern Language Association (MLA)
Barraza-Garza, Guillermo…[et al.]. Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114731
American Medical Association (AMA)
Barraza-Garza, Guillermo& Castillo-Michel, Hiram& de la Rosa, Laura A.& Martínez Martínez, Alejandro& Pérez-León, Jorge A.& Cotte, Marine…[et al.]. Infrared Spectroscopy as a Tool to Study the Antioxidant Activity of Polyphenolic Compounds in Isolated Rat Enterocytes. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114731
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114731