![](/images/graphics-bg.png)
A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma
Joint Authors
Estruch, Ramón
Lamuela-Raventós, Rosa M.
Colmán-Martínez, Mariel
Martínez-Huélamo, Miriam
Miralles, Esther
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
A simple and accurate reversed phase high-performance liquid chromatography coupled with diode array detector (HPLC-DAD) method for simultaneously determining and quantifying the antioxidants carotenes, xanthophylls, and retinol in human plasma is presented in this paper.
Compounds were extracted with hexane, a C30 column, and a mobile phase of methanol, methyl tert-butyl ether, and water were used for the separation of the compounds.
A total of 8 carotenoids, 3 Z- β -carotene isomers, and 1 fat-soluble vitamin (retinol) were resolved within 72 min at a flow rate of 0.6 mL/min.
Detection was achieved at 450 nm for carotenoids and 330 nm for retinol.
To evaluate the effectiveness of themethod, it has been applied to an intervention study conducted on eight volunteers.
Results.
Limits of detection were between 0.1 μ g/mL for lycopene and astaxanthin and 1.3 μ g/mL for 15-Z- β -carotene.
Recoveries were ranged between 89% and 113% for α -carotene and astaxanthin, respectively.
Accuracy was between 90.7% and 112.2% and precision was between 1% and 15% RSD.
In human plasma samples compounds studied were identified besides three lycopene isomers, demonstrated to be suitable for application in dietary intervention studies.
Conclusions.
Due to its accuracy, precision, selectivity, and reproducibility, this method is suitable to dietary habits and/or antioxidants status studies.
American Psychological Association (APA)
Colmán-Martínez, Mariel& Martínez-Huélamo, Miriam& Miralles, Esther& Estruch, Ramón& Lamuela-Raventós, Rosa M.. 2015. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
Modern Language Association (MLA)
Colmán-Martínez, Mariel…[et al.]. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
American Medical Association (AMA)
Colmán-Martínez, Mariel& Martínez-Huélamo, Miriam& Miralles, Esther& Estruch, Ramón& Lamuela-Raventós, Rosa M.. A New Method to Simultaneously Quantify the Antioxidants: Carotenes, Xanthophylls, and Vitamin A in Human Plasma. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114740
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114740