Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice
Joint Authors
Nagahisa, Hiroshi
Miyata, Hirofumi
Okabe, Kazuma
Iuchi, Yoshihito
Fujii, Junichi
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Cu/Zn superoxide dismutase (SOD1) knockout (KO) mice are known as an aging model in some aspects, but the damage and regeneration process of each fiber type have not been sufficiently studied.
In this study, we investigated the damage and satellite cell state of the gastrocnemius muscle in SOD1 KO mice (6 months old) using immunohistochemical staining and real-time RT-PCR.
The proportion of central nuclei-containing Type IIx/b fibers in the deep and superficial portions of the gastrocnemius muscle was significantly higher in SOD1 KO than control mice.
The number of satellite cells per muscle fiber decreased in all muscle fiber types in the deep portion of the gastrocnemius muscle in SOD1 KO mice.
In addition, the mRNA expression levels of Pax7 and myogenin, which are expressed in satellite cells in the activation, proliferation, and differentiation states, significantly increased in the gastrocnemius muscle of SOD1 KO mice.
Furthermore, mRNA of myosin heavy chain-embryonic, which is expressed in the early phase of muscle regeneration, significantly increased in SOD1 KO mice.
It was suggested that muscle is damaged by reactive oxygen species produced in the mitochondrial intermembrane space in Type IIxb fibers, accelerating the proliferation and differentiation of satellite cells through growth factors in SOD1 KO mice.
American Psychological Association (APA)
Nagahisa, Hiroshi& Okabe, Kazuma& Iuchi, Yoshihito& Fujii, Junichi& Miyata, Hirofumi. 2015. Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114754
Modern Language Association (MLA)
Nagahisa, Hiroshi…[et al.]. Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1114754
American Medical Association (AMA)
Nagahisa, Hiroshi& Okabe, Kazuma& Iuchi, Yoshihito& Fujii, Junichi& Miyata, Hirofumi. Characteristics of Skeletal Muscle Fibers of SOD1 Knockout Mice. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1114754
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114754