GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat
Joint Authors
Gao, Xiaoqing
Yang, Chaoxian
Du, Jie
Deng, Li
Wang, Yun
Yin, Ling
Yuan, Qionglan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-26
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Multiple sclerosis (MS) is an autoimmune disease in the CNS.
The current immunomodulating drugs for MS do not effectively prevent the progressive neurological decline.
Neural stem cells (NSCs) transplantation has been proven to promote repair and functional recovery of experimental allergic encephalomyelitis (EAE) animal model for MS, and glial cell line-derived neurotrophic factor (GDNF) has also been found to have capability of promoting axonal regeneration and remyelination of regenerating axons.
In the present study, to assess whether GDNF would enhance therapeutic effect of NSCs for EAE, GDNF gene-modified NSCs (GDNF/NSCs) and native NSCs were transplanted into each lateral ventricle of rats at 10 days and rats were sacrificed at 60 days after EAE immunization.
We found that NSCs significantly reduced the clinical signs, and GDNF gene-modification further promoted functional recovery.
GDNF/NSCs more profoundly suppressed brain inflammation and improved density of myelin compared with NSCs.
The survival of GDNF/NSCs was significantly higher than that of transplanted NSCs.
Transplanted GDNF/NSCs, in contrast to NSCs, differentiated into more neurons and oligodendrocytes.
Moreover, the mRNA expression of oligodendrocyte lineage cells in rats with GDNF/NSCs was significantly increased compared to rats with NSCs.
These results suggest that GDNF enhances therapeutic efficiency of NSCs-based therapy for EAE.
American Psychological Association (APA)
Gao, Xiaoqing& Deng, Li& Wang, Yun& Yin, Ling& Yang, Chaoxian& Du, Jie…[et al.]. 2016. GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116043
Modern Language Association (MLA)
Gao, Xiaoqing…[et al.]. GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116043
American Medical Association (AMA)
Gao, Xiaoqing& Deng, Li& Wang, Yun& Yin, Ling& Yang, Chaoxian& Du, Jie…[et al.]. GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116043
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116043