tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis
Joint Authors
Sun, Cheuk-Kwan
Leu, Steve
Day, Yuan-Ji
Yip, Hon-Kan
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-23, 23 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-16
Country of Publication
Egypt
No. of Pages
23
Abstract EN
We examined the role of tissue plasminogen activator- (tPA-) matrix metalloproteinase- (MMP-) 9 in mobilizing endothelial progenitor cells (EPCs) from bone marrow to circulation and critical limb ischemia (CLI) region.
Male C57BL/6J mice having been irradiated were categorized into wild-type mice (WT) receiving WT bone marrow cell (BMC) transfusion (group 1), WT mice receiving MMP-9 knockout (MMP-9−/−) BMC (group 2), MMP-9−/− receiving MMP-9−/− BMC (group 3), and MMP-9−/− receiving WT BMC (group 4), each of which was subdivided into sham control (SC), CLI, SC-tPA, and CLI-tPA.
In groups 1 and 4, by post-CLI 18 h and day 14, circulating EPC (C-kit+/CD31+, Sca-1+/KDR+) levels were highest in CLI-tPA subgroup.
In groups 2 and 3, EPC levels did not differ among all subgroups.
The EPC levels in bone marrow were higher in groups 2 and 3 than those in groups 1 and 4.
By day 14, in animals with CLI, expression levels of proangiogenic factors (CXCR4, SDF-1α, and VEGF) showed similar trends as circulating EPC levels.
Moreover, the number of infiltrated neutrophils and macrophages in quadriceps was higher in groups 1 and 4 than groups in 2 and 3.
In conclusion, tPA-MMP-9 axis plays a crucial role in EPC mobilization and angiogenesis in experimental CLI.
American Psychological Association (APA)
Leu, Steve& Day, Yuan-Ji& Sun, Cheuk-Kwan& Yip, Hon-Kan. 2016. tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis. Stem Cells International،Vol. 2016, no. 2016, pp.1-23.
https://search.emarefa.net/detail/BIM-1116758
Modern Language Association (MLA)
Leu, Steve…[et al.]. tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-23.
https://search.emarefa.net/detail/BIM-1116758
American Medical Association (AMA)
Leu, Steve& Day, Yuan-Ji& Sun, Cheuk-Kwan& Yip, Hon-Kan. tPA-MMP-9 Axis Plays a Pivotal Role in Mobilization of Endothelial Progenitor Cells from Bone Marrow to Circulation and Ischemic Region for Angiogenesis. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-23.
https://search.emarefa.net/detail/BIM-1116758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116758